Pseudomonas aeruginosa PAO1, PA4295 (fppA)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA4295
Name
fppA
Replicon chromosome
Genomic location 4819928 - 4820410 (+ strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_252985.1
GI 15599491
Affymetrix PA4295_at
DNASU PaCD00007862
Entrez 881657
GenBank AAG07683.1
INSDC AAG07683.1
NCBI Locus Tag PA4295
protein_id(GenBank) gb|AAG07683.1|AE004845_9|gnl|PseudoCAP|PA4295
TIGR NTL03PA04296
UniParc UPI00000C5CA0
UniProtKB Acc Q9HWA5
UniProtKB ID Q9HWA5_PSEAE
UniRef100 UniRef100_Q9HWA5
UniRef50 UniRef50_Q9HWA5
UniRef90 UniRef90_Q9HWA5

Product

Feature Type CDS
Coding Frame 1
Product Name
Flp prepilin peptidase A, FppA
Product Name Confidence: Class 1
Synonyms Flp prepilin peptidase A, FppA
Evidence for Translation
Charge (pH 7) 8.10
Kyte-Doolittle Hydrophobicity Value 0.821
Molecular Weight (kDa) 17.5
Isoelectric Point (pI) 9.87

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Membrane Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Virulence Evidence

Source 1
Database PseudoCAP
Category Not available / Not known
Evidence ECO:0000315
mutant phenotype evidence used in manual assertion
Host Organism Populus trichocarpa
Notes
Infection model Poplar pathogenicity wilting assay
Reference 21261818

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 10 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG003896 (354 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA4295
Search term: fppA

Human Homologs

References

Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res 2005 Feb;15(2):321-9
PubMed ID: 15687295
FppA, a novel Pseudomonas aeruginosa prepilin peptidase involved in assembly of type IVb pili.
de Bentzmann S, Aurouze M, Ball G, Filloux A
J Bacteriol 2006 Jul;188(13):4851-60
PubMed ID: 16788194