Pseudomonas aeruginosa PAO1, PA4397 (panE)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse View in GBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
GCF_000006765.1|latest
Locus Tag
PA4397
Name
panE
Synonym: abpA
Replicon chromosome
Genomic location 4927036 - 4927947 (+ strand)
Transposon Mutants 3 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_253087.1
GI 15599593
Affymetrix PA4397_panE_at
Entrez 881354
GenBank AAG07785.1
INSDC AAG07785.1
NCBI Locus Tag PA4397
protein_id(GenBank) gb|AAG07785.1|AE004855_7|gnl|PseudoCAP|PA4397
TIGR NTL03PA04398
UniParc UPI00001312D6
UniProtKB Acc Q9HW09
UniProtKB ID PANE_PSEAE
UniRef100 UniRef100_Q9HW09
UniRef50 UniRef50_Q9HW09
UniRef90 UniRef90_Q9HW09

Product

Feature Type CDS
Coding Frame 1
Product Name
ketopantoate reductase
Product Name Confidence: Class 2
Synonyms thiamin biosynthesis protein
alternative pyrimidine biosynthesis protein
Evidence for Translation
Charge (pH 7) 7.22
Kyte-Doolittle Hydrophobicity Value -0.218
Molecular Weight (kDa) 33.1
Isoelectric Point (pI) 8.82

Subcellular localization

Individual Mappings
Localization Confidence PMID
Unknown Class 3
Unknown Class 3
Additional evidence for subcellular localization

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
5ZIX OXIDOREDUCTASE 03/17/18 Crystal structure of Ketopantoate reductase from Pseudomonas aeruginosa bound to NADP+ PSEUDOMONAS AERUGINOSA PAO1 2.57 X-RAY DIFFRACTION 100.0
5ZIK OXIDOREDUCTASE 03/16/18 Crystal structure of Ketopantoate reductase from Pseudomonas aeruginosa PSEUDOMONAS AERUGINOSA PAO1 2.45 X-RAY DIFFRACTION 100.0

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 288 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG003987 (551 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA4397
Search term: panE

References

apbA, a new genetic locus involved in thiamine biosynthesis in Salmonella typhimurium.
Downs DM, Petersen L
J. Bacteriol. 1994 Aug;176(16):4858-64
PubMed ID: 7519593
ApbA, the ketopantoate reductase enzyme of Salmonella typhimurium is required for the synthesis of thiamine via the alternative pyrimidine biosynthetic pathway.
Frodyma ME, Downs D
J. Biol. Chem. 1998 Mar;273(10):5572-6
PubMed ID: 9488683
The panE gene, encoding ketopantoate reductase, maps at 10 minutes and is allelic to apbA in Salmonella typhimurium.
Frodyma ME, Downs D
J. Bacteriol. 1998 Sep;180(17):4757-9
PubMed ID: 9721324