Pseudomonas aeruginosa PAO1, PA4406 (lpxC)

Gene Feature Overview

Strain	Pseudomonas aeruginosa PAO1 (Stover et al., 2000) GCF_000006765.1|latest
Locus Tag	PA4406
Name	lpxC Synonym: envA Synonym: asmB
Replicon	chromosome
Genomic location	4938276 - 4939187 (- strand)
Transposon Mutants in orthologs	1 transposon mutants in orthologs

Cross-References

RefSeq	NP_253096.1
GI	15599602
Affymetrix	PA4406_lpxC_at
DNASU	PaCD00007909
Entrez	881292
GenBank	AAG07794.1
INSDC	AAG07794.1
NCBI Locus Tag	PA4406
protein_id(GenBank)	gb|AAG07794.1|AE004856_5|gnl|PseudoCAP|PA4406
TIGR	NTL03PA04407
UniParc	UPI000012E8E2
UniProtKB Acc	P47205
UniProtKB ID	LPXC_PSEAE
UniRef100	UniRef100_B7UZI4
UniRef50	UniRef50_Q6D0I9
UniRef90	UniRef90_Q87WZ1

Product

Feature Type	CDS
Coding Frame	1
Product Name	UDP-3-O-acyl-N-acetylglucosamine deacetylase Product Name Confidence: Class 1
Synonyms	EnvA protein
Evidence for Translation	Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602). Detected by MALDI-TOF/TOF (PMID:19333994).
Charge (pH 7)	-7.82
Kyte-Doolittle Hydrophobicity Value	-0.066
Molecular Weight (kDa)	33.4
Isoelectric Point (pI)	5.00

Subcellular localization

Individual Mappings	Localization Confidence PMID Cytoplasmic Class 3 20472543
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession	Header	Accession Date	Compound	Source	Resolution	Method	Percent Identity
6MOD	HYDROLASE/INHIBITOR	10/04/18	Co-Crystal structure of P. aeruginosa LpxC-50432 complex	Pseudomonas aeruginosa	1.85	X-RAY DIFFRACTION	99.7
7K99	HYDROLASE	09/28/20	Crystal Structure of P. aeruginosa LpxC with N-Hydroxyformamide inhibitor 19	Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)	1.9	X-RAY DIFFRACTION	100.0
4LCG	HYDROLASE/ANTIBIOTIC	06/21/13	Crystal structure of the Pseudomonas aeruginosa LPXC/LPC-050 complex	Pseudomonas aeruginosa	1.568	X-RAY DIFFRACTION	99.7
6C9C	hydrolase/hydrolase inhibitor	01/26/18	Crystal structure of LpxC from Pseudomonas aeruginosa in complex with racemic ligand PT803	Pseudomonas aeruginosa (strain UCBPP-PA14)	2	X-RAY DIFFRACTION	100.0
4FW7	HYDROLASE/HYDROLASE INHIBITOR	06/30/12	Crystal Structure of the LpxC in complex with N-[(2S,3R)-3-HYDROXY-1-(HYDROXYAMINO)-1-OXOBUTAN-2-YL]BIPHENYL-4-CARBOXAMIDE inhibitor	Pseudomonas aeruginosa	1.7	X-RAY DIFFRACTION	99.7
6DUI	hydrolase/hydrolase inhibitor	06/20/18	Crystal structure of LpxC from Pseudomonas aeruginosa in complex with PT801	Pseudomonas aeruginosa (strain LESB58)	1.55	X-RAY DIFFRACTION	100.0
7CID	HYDROLASE	07/07/20	Crystal structure of P.aeruginosa LpxC in complex with inhibitor	Pseudomonas aeruginosa PAO1	2.49	X-RAY DIFFRACTION	99.7
5UPG	HYDROLASE	02/02/17	Crystal structure of LpxC from Pseudomonas aeruginosa in complex with PF-5081090	Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)	1.7	X-RAY DIFFRACTION	100.0
6CAX	hydrolase/hydrolase inhibitor	02/01/18	Crystal structure of LpxC from Pseudomonas aeruginosa in complex with PT805	Pseudomonas aeruginosa	1.25	X-RAY DIFFRACTION	100.0
6E54	hydrolase/hydrolase inhibitor	07/19/18	Crystal structure of LpxC from Pseudomonas aeruginosa in complex with ligand PT802	Pseudomonas aeruginosa	1.65	X-RAY DIFFRACTION	100.0
8E4A	BIOSYNTHETIC PROTEIN	08/17/22	Pseudomonas LpxC in complex with LPC-233	Pseudomonas aeruginosa	2.034	X-RAY DIFFRACTION	99.7
5VWM	Hydrolase/Hydrolase Inhibitor	05/22/17	Crystal structure of UDP-3-O-[3-hydroxymyristoyl] N-acetylglucosamine deacetylase (LpxC) from Pseudomonas aeruginosa in complex with CHIR-090 inhibitor	Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)	1.8	X-RAY DIFFRACTION	100.0
6MO5	HYDROLASE/INHIBITOR	10/04/18	Co-Crystal structure of P. aeruginosa LpxC-50228 complex	Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)	1.851	X-RAY DIFFRACTION	99.7
5U3B	HYDROLASE	12/01/16	Pseudomonas aeruginosa LpxC in complex with NVS-LPXC-01	Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)	2	X-RAY DIFFRACTION	100.0
5DRR	hydrolase/hydrolase inhibitor	09/16/15	Crystal structure of the Pseudomonas aeruginosa LpxC/LPC-058 complex	Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)	1.59	X-RAY DIFFRACTION	99.7
6MAE	HYDROLASE	08/27/18	CHAIN A. UDP-3-O-[3-hydroxymyristoyl] N-acetylglucosamine deacetylase PA-LPXC Complexed with (R)-3-((S)-3-(4-(cyclopropylethynyl)phenyl)-2-oxooxazolidin-5-yl)-N-hydroxy-2-methyl-2-(methylsulfonyl)propenamide	Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)	1.8	X-RAY DIFFRACTION	100.0
7K9A	HYDROLASE	09/29/20	Crystal Structure of P. aeruginosa LpxC with N-Hydroxyformamide inhibitor	Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)	2	X-RAY DIFFRACTION	100.0
5N8C	HYDROLASE	02/23/17	Crystal structure of Pseudomonas aeruginosa LpxC complexed with inhibitor	Pseudomonas aeruginosa	1.9	X-RAY DIFFRACTION	99.7
6MO4	HYDROLASE/INHIBITOR	10/04/18	Co-Crystal structure of P. aeruginosa LpxC-50067 complex	Pseudomonas aeruginosa PAO1	1.844	X-RAY DIFFRACTION	100.0
4LCF	HYDROLASE/ANTIBIOTIC	06/21/13	Crystal structure of the Pseudomonas aeruginosa LPXC/LPC-014 complex	Pseudomonas aeruginosa	1.599	X-RAY DIFFRACTION	99.7

Drugs Targeting this Protein

Identified by Diamond using e-value cutoff of 0.0001 and returning alignments that span 100% of the query sequence and that have more than 95% identity.

Drug Name	Source Accession	Source DB	Version	Target Accession	Target Description	Percent Identity	Alignment Length	E-Value
(2R)-N-hydroxy-3-naphthalen-2-yl-2-[(naphthalen-2-ylsulfonyl)amino]propanamide	DB07861	DrugBank	5.1.4	P47205	UDP-3-O-[3-hydroxymyristoyl] N-acetylglucosamine deacetylase	100.0	303	8.5e-167

Pathogen Association Analysis

Results

Common Found in both pathogen and nonpathogenic strains Hits to this gene were found in 387 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database	View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group	POG003996 (535 members)
Putative Inparalogs	None Found

Interactions

STRING database

Search for predicted protein-protein interactions using: Search term: PA4406 Search term: lpxC Search term: UDP-3-O-acyl-N-acetylglucosamine deacetylase

Human Homologs

References

Sequence analysis, transcriptional organization, and insertional mutagenesis of the envA gene of Escherichia coli. Beall B, Lutkenhaus J J Bacteriol 1987 Dec;169(12):5408-15 PubMed ID: 2824434

The envA permeability/cell division gene of Escherichia coli encodes the second enzyme of lipid A biosynthesis. UDP-3-O-(R-3-hydroxymyristoyl)-N-acetylglucosamine deacetylase. Young K, Silver LL, Bramhill D, Cameron P, Eveland SS, Raetz CR, Hyland SA, Anderson MS J Biol Chem 1995 Dec 22;270(51):30384-91 PubMed ID: 8530464

asmB, a suppressor locus for assembly-defective OmpF mutants of Escherichia coli, is allelic to envA (lpxC). Kloser AW, Laird MW, Misra R J Bacteriol 1996 Sep;178(17):5138-43 PubMed ID: 8752330

Cloning, expression, and purification of UDP-3-O-acyl-GlcNAc deacetylase from Pseudomonas aeruginosa: a metalloamidase of the lipid A biosynthesis pathway. Hyland SA, Eveland SS, Anderson MS J Bacteriol 1997 Mar;179(6):2029-37 PubMed ID: 9068651

Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen. Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM Proteomics 2009 Apr;9(7):1901-15 PubMed ID: 19333994