Pseudomonas aeruginosa PAO1, PA4410 (ddlB)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA4410
Name
ddlB
Replicon chromosome
Genomic location 4942677 - 4943636 (- strand)
Transposon Mutants 1 transposon mutants in PAO1
Transposon Mutants in orthologs 2 transposon mutants in orthologs

Cross-References

RefSeq NP_253100.1
GI 15599606
Affymetrix PA4410_ddlB_at
DNASU PaCD00007911
Entrez 881282
GenBank AAG07798.1
INSDC AAG07798.1
NCBI Locus Tag PA4410
protein_id(GenBank) gb|AAG07798.1|AE004856_9|gnl|PseudoCAP|PA4410
TIGR NTL03PA04411
UniParc UPI00000C4ED3
UniProtKB Acc Q9LCT6
UniProtKB ID DDLB_PSEAE
UniRef100 UniRef100_Q9LCT6
UniRef50 UniRef50_P07862
UniRef90 UniRef90_Q9LCT6

Product

Feature Type CDS
Coding Frame 1
Product Name
D-alanine--D-alanine ligase
Product Name Confidence: Class 2
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -11.18
Kyte-Doolittle Hydrophobicity Value -0.005
Molecular Weight (kDa) 34.4
Isoelectric Point (pI) 4.66

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Cytoplasmic Class 2
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 604 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG004000 (530 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA4410
Search term: ddlB

Human Homologs

References

Vancomycin resistance: structure of D-alanine:D-alanine ligase at 2.3 A resolution.
Fan C, Moews PC, Walsh CT, Knox JR
Science 1994 Oct 21;266(5184):439-43
PubMed ID: 7939684
D-alanine:D-alanine ligase: phosphonate and phosphinate intermediates with wild type and the Y216F mutant.
Fan C, Park IS, Walsh CT, Knox JR
Biochemistry 1997 Mar 4;36(9):2531-8
PubMed ID: 9054558