Strain |
Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest |
Locus Tag |
PA4411
|
Name |
murC
|
Replicon | chromosome |
Genomic location | 4943633 - 4945075 (- strand) |
RefSeq | NP_253101.1 |
GI | 15599607 |
Affymetrix | PA4411_murC_at |
Entrez | 881283 |
GenBank | AAG07799.1 |
INSDC | AAG07799.1 |
NCBI Locus Tag | PA4411 |
protein_id(GenBank) | gb|AAG07799.1|AE004856_10|gnl|PseudoCAP|PA4411 |
TIGR | NTL03PA04412 |
UniParc | UPI000012F96B |
UniProtKB Acc | Q9HW02 |
UniProtKB ID | MURC_PSEAE |
UniRef100 | UniRef100_B7UZI9 |
UniRef50 | UniRef50_Q87SG3 |
UniRef90 | UniRef90_A6VB84 |
Feature Type | CDS |
Coding Frame | 1 |
Product Name |
UDP-N-acetylmuramate--alanine ligase
|
Synonyms | |
Evidence for Translation |
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Detected by MALDI-TOF/TOF (PMID:19333994).
|
Charge (pH 7) | -6.22 |
Kyte-Doolittle Hydrophobicity Value | 0.013 |
Molecular Weight (kDa) | 51.9 |
Isoelectric Point (pI) | 6.10 |
Individual Mappings | |
Additional evidence for subcellular localization |
Accession | Header | Accession Date | Compound | Source | Resolution | Method | Percent Identity |
8EGM | LIGASE | 09/12/22 | Crystal Structure of UDP-N-acetylmuramate-L-alanine ligase (UDP-N-acetylmuramoyl-L-alanine synthetase, MurC) Pseudomonas aeruginosa in complex with compound AZ13644908 | Pseudomonas aeruginosa PAO1 | 2.2 | X-RAY DIFFRACTION | 100.0 |
6X9F | LIGASE | 06/02/20 | Pseudomonas aeruginosa MurC with AZ8074 | Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) | 2.35 | X-RAY DIFFRACTION | 100.0 |
5VVW | LIGASE | 05/21/17 | Structure of MurC from Pseudomonas aeruginosa | Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) | 2.3 | X-RAY DIFFRACTION | 100.0 |
6X9N | LIGASE | 06/03/20 | Pseudomonas aeruginosa MurC with AZ5595 | Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) | 2.25 | X-RAY DIFFRACTION | 100.0 |
8DOF | LIGASE | 07/13/22 | Pseudomonas aeruginosa MurC with WYH9-2-P - OSA_001044 | Pseudomonas aeruginosa | 2.6 | X-RAY DIFFRACTION | 100.0 |
Results |
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 583 genera
|
Pseudomonas Ortholog Database | View orthologs at Pseudomonas Ortholog Database |
Pseudomonas Ortholog Group |
POG004001 (535 members) |
Putative Inparalogs | None Found |
STRING database | Search for predicted protein-protein interactions using:
Search term: PA4411
Search term: murC
Search term: UDP-N-acetylmuramate--alanine ligase
|
Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res 2005 Feb;15(2):321-9
PubMed ID: 15687295
|
Cloning, over-expression and purification of Pseudomonas aeruginosa murC encoding uridine diphosphate N-acetylmuramate: L-alanine ligase.
El Zoeiby A, Sanschagrin F, Lamoureux J, Darveau A, Levesque RC
FEMS Microbiol Lett 2000 Feb 15;183(2):281-8
PubMed ID: 10675598
|
Systematic sequencing of the Escherichia coli genome: analysis of the 0-2.4 min region.
Yura T, Mori H, Nagai H, Nagata T, Ishihama A, Fujita N, Isono K, Mizobuchi K, Nakata A
Nucleic Acids Res 1992 Jul 11;20(13):3305-8
PubMed ID: 1630901
|
Over-production, purification and properties of the uridine-diphosphate-N-acetylmuramate:L-alanine ligase from Escherichia coli.
Liger D, Masson A, Blanot D, van Heijenoort J, Parquet C
Eur J Biochem 1995 May 15;230(1):80-7
PubMed ID: 7601127
|
Study of the overproduced uridine-diphosphate-N-acetylmuramate:L-alanine ligase from Escherichia coli.
Liger D, Masson A, Blanot D, van Heijenoort J, Parquet C
Microb Drug Resist 1996 Spring;2(1):25-7
PubMed ID: 9158719
|
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994
|