Pseudomonas aeruginosa PAO1, PA4411 (murC)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA4411
Name
murC
Replicon chromosome
Genomic location 4943633 - 4945075 (- strand)

Cross-References

RefSeq NP_253101.1
GI 15599607
Affymetrix PA4411_murC_at
Entrez 881283
GenBank AAG07799.1
INSDC AAG07799.1
NCBI Locus Tag PA4411
protein_id(GenBank) gb|AAG07799.1|AE004856_10|gnl|PseudoCAP|PA4411
TIGR NTL03PA04412
UniParc UPI000012F96B
UniProtKB Acc Q9HW02
UniProtKB ID MURC_PSEAE
UniRef100 UniRef100_B7UZI9
UniRef50 UniRef50_Q87SG3
UniRef90 UniRef90_A6VB84

Product

Feature Type CDS
Coding Frame 1
Product Name
UDP-N-acetylmuramate--alanine ligase
Product Name Confidence: Class 2
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Detected by MALDI-TOF/TOF (PMID:19333994).
Charge (pH 7) -6.22
Kyte-Doolittle Hydrophobicity Value 0.013
Molecular Weight (kDa) 51.9
Isoelectric Point (pI) 6.10

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
8EGM LIGASE 09/12/22 Crystal Structure of UDP-N-acetylmuramate-L-alanine ligase (UDP-N-acetylmuramoyl-L-alanine synthetase, MurC) Pseudomonas aeruginosa in complex with compound AZ13644908 Pseudomonas aeruginosa PAO1 2.2 X-RAY DIFFRACTION 100.0
6X9F LIGASE 06/02/20 Pseudomonas aeruginosa MurC with AZ8074 Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) 2.35 X-RAY DIFFRACTION 100.0
5VVW LIGASE 05/21/17 Structure of MurC from Pseudomonas aeruginosa Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) 2.3 X-RAY DIFFRACTION 100.0
6X9N LIGASE 06/03/20 Pseudomonas aeruginosa MurC with AZ5595 Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) 2.25 X-RAY DIFFRACTION 100.0
8DOF LIGASE 07/13/22 Pseudomonas aeruginosa MurC with WYH9-2-P - OSA_001044 Pseudomonas aeruginosa 2.6 X-RAY DIFFRACTION 100.0

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 583 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG004001 (535 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA4411
Search term: murC

Human Homologs

References

Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res 2005 Feb;15(2):321-9
PubMed ID: 15687295
Cloning, over-expression and purification of Pseudomonas aeruginosa murC encoding uridine diphosphate N-acetylmuramate: L-alanine ligase.
El Zoeiby A, Sanschagrin F, Lamoureux J, Darveau A, Levesque RC
FEMS Microbiol Lett 2000 Feb 15;183(2):281-8
PubMed ID: 10675598
Systematic sequencing of the Escherichia coli genome: analysis of the 0-2.4 min region.
Yura T, Mori H, Nagai H, Nagata T, Ishihama A, Fujita N, Isono K, Mizobuchi K, Nakata A
Nucleic Acids Res 1992 Jul 11;20(13):3305-8
PubMed ID: 1630901
Over-production, purification and properties of the uridine-diphosphate-N-acetylmuramate:L-alanine ligase from Escherichia coli.
Liger D, Masson A, Blanot D, van Heijenoort J, Parquet C
Eur J Biochem 1995 May 15;230(1):80-7
PubMed ID: 7601127
Study of the overproduced uridine-diphosphate-N-acetylmuramate:L-alanine ligase from Escherichia coli.
Liger D, Masson A, Blanot D, van Heijenoort J, Parquet C
Microb Drug Resist 1996 Spring;2(1):25-7
PubMed ID: 9158719
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994