Pseudomonas aeruginosa PAO1, PA4414 (murD)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA4414
Name
murD
Replicon chromosome
Genomic location 4947330 - 4948676 (- strand)
Transposon Mutants 1 transposon mutants in PAO1

Cross-References

RefSeq NP_253104.1
GI 15599610
Affymetrix PA4414_murD_at
Entrez 881268
GenBank AAG07802.1
INSDC AAG07802.1
NCBI Locus Tag PA4414
protein_id(GenBank) gb|AAG07802.1|AE004856_13|gnl|PseudoCAP|PA4414
TIGR NTL03PA04415
UniParc UPI00000C5D03
UniProtKB Acc Q9HVZ9
UniProtKB ID MURD_PSEAE
UniRef100 UniRef100_Q9HVZ9
UniRef50 UniRef50_Q9HVZ9
UniRef90 UniRef90_Q9HVZ9

Product

Feature Type CDS
Coding Frame 1
Product Name
UDP-N-acetylmuramoylalanine--D-glutamate ligase
Product Name Confidence: Class 2
Synonyms D-glutamate-adding enzyme
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -6.27
Kyte-Doolittle Hydrophobicity Value 0.125
Molecular Weight (kDa) 48.1
Isoelectric Point (pI) 5.70

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 2
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
8DP2 LIGASE 07/14/22 Crystal Structure of UDP-N-acetylmuramoylalanine--D-glutamate ligase (MurD) from Pseudomonas aeruginosa PAO1 in complex with UMA (Uridine-5'-diphosphate-N-acetylmuramoyl-L-Alanine) Pseudomonas aeruginosa PAO1 1.6 X-RAY DIFFRACTION 100.0
7U35 LIGASE 02/25/22 Crystal Structure of UDP-N-acetylmuramoylalanine--D-glutamate ligase (MurD) from Pseudomonas aeruginosa PAO1 in complex with ADP Pseudomonas aeruginosa PAO1 1.95 X-RAY DIFFRACTION 100.0
7SY9 LIGASE 11/24/21 Crystal Structure of UDP-N-acetylmuramoylalanine--D-glutamate ligase (MurD) from Pseudomonas aeruginosa PAO1 Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) 2.75 X-RAY DIFFRACTION 100.0

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 574 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG004004 (535 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA4414
Search term: murD

Human Homologs

References

Nucleotide sequence of the murD gene encoding the UDP-MurNAc-L-Ala-D-Glu synthetase of Escherichia coli.
Mengin-Lecreulx D, van Heijenoort J
Nucleic Acids Res 1990 Jan 11;18(1):183
PubMed ID: 2129548
Over-production, purification and properties of the uridine diphosphate N-acetylmuramoyl-L-alanine:D-glutamate ligase from Escherichia coli.
Pratviel-Sosa F, Mengin-Lecreulx D, van Heijenoort J
Eur J Biochem 1991 Dec 18;202(3):1169-76
PubMed ID: 1765076
Crystal structure of UDP-N-acetylmuramoyl-L-alanine:D-glutamate ligase from Escherichia coli.
Bertrand JA, Auger G, Fanchon E, Martin L, Blanot D, van Heijenoort J, Dideberg O
EMBO J 1997 Jun 16;16(12):3416-25
PubMed ID: 9218784