Pseudomonas aeruginosa PAO1, PA4423

Gene Feature Overview

Strain	Pseudomonas aeruginosa PAO1 (Stover et al., 2000) GCF_000006765.1|latest
Locus Tag	PA4423
Name	Synonym: yraM
Replicon	chromosome
Genomic location	4957709 - 4959523 (+ strand)
Transposon Mutants	2 transposon mutants in PAO1
Transposon Mutants in orthologs	1 transposon mutants in orthologs
Comment	Localication to outer membrane vesicle confirmed in three separate studies (PMID:22909304, PMID:21751344 and PMID:26303878).

Cross-References

RefSeq	NP_253113.1
GI	15599619
Affymetrix	PA4423_at
Entrez	881236
GenBank	AAG07811.1
INSDC	AAG07811.1
NCBI Locus Tag	PA4423
protein_id(GenBank)	gb|AAG07811.1|AE004857_2|gnl|PseudoCAP|PA4423
TIGR	NTL03PA04424
UniParc	UPI00000C5D06
UniProtKB Acc	Q9HVZ2
UniProtKB ID	Q9HVZ2_PSEAE
UniRef100	UniRef100_Q9HVZ2
UniRef50	UniRef50_K9NRD2
UniRef90	UniRef90_Q9HVZ2

Product

Feature Type	CDS
Coding Frame	1
Product Name	conserved hypothetical protein Product Name Confidence: Class 4
Synonyms
Evidence for Translation	Detected by MALDI-TOF/TOF (PMID:19333994). Detected using LC-MS/MS (65.63 kDa, PMID:21751344) Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7)	0.61
Kyte-Doolittle Hydrophobicity Value	-0.284
Molecular Weight (kDa)	65.6
Isoelectric Point (pI)	7.20

Subcellular localization

Individual Mappings	Localization Confidence PMID Outer Membrane Vesicle Class 1 Periplasmic Class 1 Cytoplasmic Membrane Class 3 20472543
Additional evidence for subcellular localization	Detected in a periplasmic protein preparation by spheroplasting by lysozyme and sucrose.(PMID:19333994)

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results

Common Found in both pathogen and nonpathogenic strains Hits to this gene were found in 104 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database	View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group	POG004013 (536 members)
Putative Inparalogs	None Found

Interactions

STRING database

Search for predicted protein-protein interactions using: Search term: PA4423

Human Homologs

References

Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen. Lewenza S, Gardy JL, Brinkman FS, Hancock RE Genome Res 2005 Feb;15(2):321-9 PubMed ID: 15687295

Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen. Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM Proteomics 2009 Apr;9(7):1901-15 PubMed ID: 19333994

Proteomic analysis of outer membrane vesicles derived from Pseudomonas aeruginosa. Choi DS, Kim DK, Choi SJ, Lee J, Choi JP, Rho S, Park SH, Kim YK, Hwang D, Gho YS Proteomics 2011 Aug;11(16):3424-9 PubMed ID: 21751344

Identification of proteins associated with the Pseudomonas aeruginosa biofilm extracellular matrix. Toyofuku M, Roschitzki B, Riedel K, Eberl L J Proteome Res 2012 Oct 5;11(10):4906-15 PubMed ID: 22909304

Proteome Profiles of Outer Membrane Vesicles and Extracellular Matrix of Pseudomonas aeruginosa Biofilms. Couto N, Schooling SR, Dutcher JR, Barber J J Proteome Res 2015 Oct 2;14(10):4207-22 PubMed ID: 26303878