Pseudomonas aeruginosa PAO1, PA4453

Gene Feature Overview

Strain	Pseudomonas aeruginosa PAO1 (Stover et al., 2000) GCF_000006765.1|latest
Locus Tag	PA4453
Name
Replicon	chromosome
Genomic location	4986152 - 4986799 (- strand)
Transposon Mutants	4 transposon mutants in PAO1
Transposon Mutants in orthologs	1 transposon mutants in orthologs
Comment	Structural similarity to 2QGU: phospholipid binding protein from Ralstonia Solanacearum (root-mean-square deviation: 1.1 angstroms)

Cross-References

RefSeq	NP_253143.1
GI	15599649
Affymetrix	PA4453_at
DNASU	PaCD00007929
Entrez	880994
GenBank	AAG07841.1
INSDC	AAG07841.1
NCBI Locus Tag	PA4453
protein_id(GenBank)	gb|AAG07841.1|AE004859_10|gnl|PseudoCAP|PA4453
TIGR	NTL03PA04454
UniParc	UPI00000C5D18
UniProtKB Acc	Q9HVW4
UniProtKB ID	Q9HVW4_PSEAE
UniRef100	UniRef100_W0YYW6
UniRef50	UniRef50_A0A080V9N0
UniRef90	UniRef90_A0A080V9N0

Product

Feature Type	CDS
Coding Frame	1
Product Name	putative phospholipid binding protein Product Name Confidence: Class 3
Synonyms
Evidence for Translation	Detected by MALDI-TOF/TOF (PMID:19333994). Detected by iTRAQ (PMID:21863142). Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602). Detected by two-dimensional gel electrophoresis (PMID:21863142). Detected by two-dimensional gel electrophoresis and mass spectral analysis(PMID:21863142).
Charge (pH 7)	3.99
Kyte-Doolittle Hydrophobicity Value	-0.258
Molecular Weight (kDa)	23.8
Isoelectric Point (pI)	9.66

Subcellular localization

Individual Mappings	Localization Confidence PMID Periplasmic Class 1 19333994 Unknown Class 3 20472543
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession	Header	Accession Date	Compound	Source	Resolution	Method	Percent Identity
6HSY	LIPID TRANSPORT	10/02/18	Two-phospholipid-bound crystal structure of the substrate-binding protein Ttg2D from Pseudomonas aeruginosa	Pseudomonas aeruginosa PAO1	2.53	X-RAY DIFFRACTION	100.0

Pathogen Association Analysis

Results

Common Found in both pathogen and nonpathogenic strains Hits to this gene were found in 128 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database	View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group	POG004042 (536 members)
Putative Inparalogs	None Found

Interactions

STRING database

Search for predicted protein-protein interactions using: Search term: PA4453 Search term: putative phospholipid binding protein

Human Homologs

References

Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen. Lewenza S, Gardy JL, Brinkman FS, Hancock RE Genome Res 2005 Feb;15(2):321-9 PubMed ID: 15687295

Isolation and characterization of toluene-sensitive mutants from the toluene-resistant bacterium Pseudomonas putida GM73. Kim K, Lee S, Lee K, Lim D J Bacteriol 1998 Jul;180(14):3692-6 PubMed ID: 9658016

Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen. Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM Proteomics 2009 Apr;9(7):1901-15 PubMed ID: 19333994

Comparisons of Two Proteomic Analyses of Non-Mucoid and Mucoid Pseudomonas aeruginosa Clinical Isolates from a Cystic Fibrosis Patient. Rao J, Damron FH, Basler M, Digiandomenico A, Sherman NE, Fox JW, Mekalanos JJ, Goldberg JB Front Microbiol 2011;2:162 PubMed ID: 21863142

Uncovering the structure and function of Pseudomonas aeruginosa periplasmic proteins by an in silico approach. Caprari S, Brandi V, Pasquadibisceglie A, Polticelli F J Biomol Struct Dyn 2020 Sep;38(15):4508-4520 PubMed ID: 31631799