Pseudomonas aeruginosa PAO1, PA4454

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA4454
Name
Synonym: yrbD
Replicon chromosome
Genomic location 4986811 - 4987284 (- strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 2 transposon mutants in orthologs

Cross-References

RefSeq NP_253144.1
GI 15599650
Affymetrix PA4454_at
DNASU PaCD00007930
Entrez 881007
GenBank AAG07842.1
INSDC AAG07842.1
NCBI Locus Tag PA4454
protein_id(GenBank) gb|AAG07842.1|AE004859_11|gnl|PseudoCAP|PA4454
TIGR NTL03PA04455
UniParc UPI00000C5D19
UniProtKB Acc Q9HVW3
UniProtKB ID Q9HVW3_PSEAE
UniRef100 UniRef100_W0YXK9
UniRef50 UniRef50_Q4KI79
UniRef90 UniRef90_W0YXK9

Product

Feature Type CDS
Coding Frame 1
Product Name
conserved hypothetical protein
Product Name Confidence: Class 4
Synonyms
Evidence for Translation
Detected using LC-MS/MS (16.56 kDa, PMID:21751344)
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -3.77
Kyte-Doolittle Hydrophobicity Value 0.274
Molecular Weight (kDa) 16.6
Isoelectric Point (pI) 4.68

Subcellular localization

Individual Mappings
Localization Confidence PMID
Unknown Class 3
Outer Membrane Vesicle Class 1
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
7CHA MEMBRANE PROTEIN 07/05/20 Cryo-EM structure of P.aeruginosa MlaFEBD with AMPPNP Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1); Pseudomonas aeruginosa PAO1 3.9 ELECTRON MICROSCOPY 100.0
7CH9 MEMBRANE PROTEIN 07/05/20 Cryo-EM structure of P.aeruginosa MlaFEBD Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) 3.5 ELECTRON MICROSCOPY 100.0
7CH8 MEMBRANE PROTEIN 07/05/20 Cryo-EM structure of P.aeruginosa MlaFEBD with ADP-V Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) 3.9 ELECTRON MICROSCOPY 100.0

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 239 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG004043 (536 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA4454

Human Homologs

References

Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res 2005 Feb;15(2):321-9
PubMed ID: 15687295
Isolation and characterization of toluene-sensitive mutants from the toluene-resistant bacterium Pseudomonas putida GM73.
Kim K, Lee S, Lee K, Lim D
J Bacteriol 1998 Jul;180(14):3692-6
PubMed ID: 9658016
Proteomic analysis of outer membrane vesicles derived from Pseudomonas aeruginosa.
Choi DS, Kim DK, Choi SJ, Lee J, Choi JP, Rho S, Park SH, Kim YK, Hwang D, Gho YS
Proteomics 2011 Aug;11(16):3424-9
PubMed ID: 21751344