Strain |
Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest |
Locus Tag |
PA4525
|
Name |
pilA
|
Replicon | chromosome |
Genomic location | 5069082 - 5069531 (- strand) |
Transposon Mutants | 2 transposon mutants in PAO1 |
Comment | Localication to outer membrane vesicle confirmed in three separate studies (PMID:22909304, PMID:21751344 and PMID:26303878). |
RefSeq | NP_253215.1 |
GI | 15599721 |
Affymetrix | PA4525_pilA_at |
DNASU | PaCD00007957 |
Entrez | 878423 |
GenBank | AAG07913.1 |
NCBI Locus Tag | PA4525 |
protein_id(GenBank) | gb|AAG07913.1|AE004866_7|gnl|PseudoCAP|PA4525 |
TIGR | NTL03PA04526 |
UniProtKB Acc | P04739 |
UniProtKB ID | FMPO_PSEAE |
Feature Type | CDS |
Coding Frame | 1 |
Product Name |
type 4 fimbrial precursor PilA
|
Synonyms |
type IV pilin |
Evidence for Translation |
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Detected using LC-MS/MS (15.51 kDa, PMID:21751344)
|
Charge (pH 7) | -0.08 |
Kyte-Doolittle Hydrophobicity Value | 0.130 |
Molecular Weight (kDa) | 15.5 |
Isoelectric Point (pI) | 6.59 |
Individual Mappings | |
Additional evidence for subcellular localization |
Source 1 | |
Database | VFDB : VF0082 |
Category | Type IVa pili |
Evidence |
|
Host Organism | |
Notes | |
Reference |
Results |
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 207 genera
|
Pseudomonas Ortholog Database | View orthologs at Pseudomonas Ortholog Database |
Pseudomonas Ortholog Group |
POG004106 (467 members) |
Putative Inparalogs | None Found |
STRING database | Search for predicted protein-protein interactions using:
Search term: PA4525
Search term: pilA
Search term: type 4 fimbrial precursor PilA
|
Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res 2005 Feb;15(2):321-9
PubMed ID: 15687295
|
Comparative studies of the amino acid and nucleotide sequences of pilin derived from Pseudomonas aeruginosa PAK and PAO.
Sastry PA, Finlay BB, Pasloske BL, Paranchych W, Pearlstone JR, Smillie LB
J Bacteriol 1985 Nov;164(2):571-7
PubMed ID: 2997119
|
Mutations in the fifth-position glutamate in Pseudomonas aeruginosa pilin affect the transmethylation of the N-terminal phenylalanine.
Macdonald DL, Pasloske BL, Paranchych W
Can J Microbiol 1993 May;39(5):500-5
PubMed ID: 8330261
|
Comparison of type IV-pilin genes of Pseudomonas aeruginosa of various habitats has uncovered a novel unusual sequence.
Spangenberg C, Fislage R, Sierralta W, Tümmler B, Römling U
FEMS Microbiol Lett 1995 Jan 15;125(2-3):265-73
PubMed ID: 7875574
|
Proteomic analysis of outer membrane vesicles derived from Pseudomonas aeruginosa.
Choi DS, Kim DK, Choi SJ, Lee J, Choi JP, Rho S, Park SH, Kim YK, Hwang D, Gho YS
Proteomics 2011 Aug;11(16):3424-9
PubMed ID: 21751344
|
Identification of proteins associated with the Pseudomonas aeruginosa biofilm extracellular matrix.
Toyofuku M, Roschitzki B, Riedel K, Eberl L
J Proteome Res 2012 Oct 5;11(10):4906-15
PubMed ID: 22909304
|
Proteome Profiles of Outer Membrane Vesicles and Extracellular Matrix of Pseudomonas aeruginosa Biofilms.
Couto N, Schooling SR, Dutcher JR, Barber J
J Proteome Res 2015 Oct 2;14(10):4207-22
PubMed ID: 26303878
|