Pseudomonas aeruginosa PAO1, PA4525 (pilA)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA4525
Name
pilA
Replicon chromosome
Genomic location 5069082 - 5069531 (- strand)
Transposon Mutants 2 transposon mutants in PAO1
CommentLocalication to outer membrane vesicle confirmed in three separate studies (PMID:22909304, PMID:21751344 and PMID:26303878).

Cross-References

RefSeq NP_253215.1
GI 15599721
Affymetrix PA4525_pilA_at
DNASU PaCD00007957
Entrez 878423
GenBank AAG07913.1
NCBI Locus Tag PA4525
protein_id(GenBank) gb|AAG07913.1|AE004866_7|gnl|PseudoCAP|PA4525
TIGR NTL03PA04526
UniProtKB Acc P04739
UniProtKB ID FMPO_PSEAE

Product

Feature Type CDS
Coding Frame 1
Product Name
type 4 fimbrial precursor PilA
Product Name Confidence: Class 1
Synonyms type IV pilin
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Detected using LC-MS/MS (15.51 kDa, PMID:21751344)
Charge (pH 7) -0.08
Kyte-Doolittle Hydrophobicity Value 0.130
Molecular Weight (kDa) 15.5
Isoelectric Point (pI) 6.59

Subcellular localization

Individual Mappings
Localization Confidence PMID
Outer Membrane Vesicle Class 1
Extracellular Class 3
Fimbrial
Note: This is based on PSORTb V3.0 secondary localization prediction
Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Virulence Evidence

Source 1
Database VFDB : VF0082
Category Type IVa pili
Evidence
Host Organism
Notes
Reference

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 207 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG004106 (467 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA4525
Search term: pilA

Human Homologs

References

Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res 2005 Feb;15(2):321-9
PubMed ID: 15687295
Comparative studies of the amino acid and nucleotide sequences of pilin derived from Pseudomonas aeruginosa PAK and PAO.
Sastry PA, Finlay BB, Pasloske BL, Paranchych W, Pearlstone JR, Smillie LB
J Bacteriol 1985 Nov;164(2):571-7
PubMed ID: 2997119
Mutations in the fifth-position glutamate in Pseudomonas aeruginosa pilin affect the transmethylation of the N-terminal phenylalanine.
Macdonald DL, Pasloske BL, Paranchych W
Can J Microbiol 1993 May;39(5):500-5
PubMed ID: 8330261
Comparison of type IV-pilin genes of Pseudomonas aeruginosa of various habitats has uncovered a novel unusual sequence.
Spangenberg C, Fislage R, Sierralta W, Tümmler B, Römling U
FEMS Microbiol Lett 1995 Jan 15;125(2-3):265-73
PubMed ID: 7875574
Proteomic analysis of outer membrane vesicles derived from Pseudomonas aeruginosa.
Choi DS, Kim DK, Choi SJ, Lee J, Choi JP, Rho S, Park SH, Kim YK, Hwang D, Gho YS
Proteomics 2011 Aug;11(16):3424-9
PubMed ID: 21751344
Identification of proteins associated with the Pseudomonas aeruginosa biofilm extracellular matrix.
Toyofuku M, Roschitzki B, Riedel K, Eberl L
J Proteome Res 2012 Oct 5;11(10):4906-15
PubMed ID: 22909304
Proteome Profiles of Outer Membrane Vesicles and Extracellular Matrix of Pseudomonas aeruginosa Biofilms.
Couto N, Schooling SR, Dutcher JR, Barber J
J Proteome Res 2015 Oct 2;14(10):4207-22
PubMed ID: 26303878