Pseudomonas aeruginosa PAO1, PA4542 (clpB)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA4542
Name
clpB
Replicon chromosome
Genomic location 5087408 - 5089972 (- strand)
Transposon Mutants 11 transposon mutants in PAO1
Transposon Mutants in orthologs 3 transposon mutants in orthologs

Cross-References

RefSeq NP_253232.1
GI 15599738
Affymetrix PA4542_clpB_at
Entrez 879457
GenBank AAG07930.1
INSDC AAG07930.1
NCBI Locus Tag PA4542
protein_id(GenBank) gb|AAG07930.1|AE004868_1|gnl|PseudoCAP|PA4542
TIGR NTL03PA04543
UniParc UPI00000C5D5D
UniProtKB Acc Q9HVN5
UniProtKB ID CLPB_PSEAE
UniRef100 UniRef100_Q9HVN5
UniRef50 UniRef50_P63284-2
UniRef90 UniRef90_Q9HVN5

Product

Feature Type CDS
Coding Frame 1
Product Name
ClpB protein
Product Name Confidence: Class 2
Synonyms
Evidence for Translation
Detected by MALDI-TOF/TOF (PMID:19333994).
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -20.58
Kyte-Doolittle Hydrophobicity Value -0.346
Molecular Weight (kDa) 95.0
Isoelectric Point (pI) 5.13

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 598 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000451 (857 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA4542
Search term: clpB
Search term: ClpB protein

Human Homologs

Ensembl 110, assembly GRCh38.p14
caseinolytic mitochondrial matrix peptidase chaperone subunit B [Source:HGNC Symbol;Acc:HGNC:30664]
E-value: 3.8e-52
Percent Identity: 36.4
Ensembl 110, assembly GRCh38.p14
caseinolytic mitochondrial matrix peptidase chaperone subunit B [Source:HGNC Symbol;Acc:HGNC:30664]
E-value: 3.8e-52
Percent Identity: 36.4
Ensembl 110, assembly GRCh38.p14
caseinolytic mitochondrial matrix peptidase chaperone subunit B [Source:HGNC Symbol;Acc:HGNC:30664]
E-value: 3.8e-52
Percent Identity: 36.4

References

Conservation of the regulatory subunit for the Clp ATP-dependent protease in prokaryotes and eukaryotes.
Gottesman S, Squires C, Pichersky E, Carrington M, Hobbs M, Mattick JS, Dalrymple B, Kuramitsu H, Shiroza T, Foster T
Proc Natl Acad Sci U S A 1990 May;87(9):3513-7
PubMed ID: 2185473
ClpB is the Escherichia coli heat shock protein F84.1.
Squires CL, Pedersen S, Ross BM, Squires C
J Bacteriol 1991 Jul;173(14):4254-62
PubMed ID: 2066329
Mutational analysis of the two ATP-binding sites in ClpB, a heat shock protein with protein-activated ATPase activity in Escherichia coli.
Kim KI, Woo KM, Seong IS, Lee ZW, Baek SH, Chung CH
Biochem J 1998 Aug 1;333 ( Pt 3)(Pt 3):671-6
PubMed ID: 9677327
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994