Pseudomonas aeruginosa PAO1, PA4544 (rluD)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA4544
Name
rluD
Synonym: yfiI
Replicon chromosome
Genomic location 5090853 - 5091815 (- strand)
Transposon Mutants 4 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_253234.1
GI 15599740
Affymetrix PA4544_rluD_at
DNASU PaCD00007967
Entrez 877896
GenBank AAG07932.1
INSDC CAA78140.1
NCBI Locus Tag PA4544
protein_id(GenBank) gb|AAG07932.1|AE004868_3|gnl|PseudoCAP|PA4544
TIGR NTL03PA04545
UniParc UPI0000134295
UniProtKB Acc P33640
UniProtKB ID RLUD_PSEAE
UniRef100 UniRef100_P33640
UniRef50 UniRef50_P33640
UniRef90 UniRef90_P33640

Product

Feature Type CDS
Coding Frame 1
Product Name
pseudouridine synthase
Product Name Confidence: Class 2
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) 2.09
Kyte-Doolittle Hydrophobicity Value -0.121
Molecular Weight (kDa) 35.6
Isoelectric Point (pI) 7.36

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 591 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG004205 (535 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA4544
Search term: rluD

Human Homologs

Ensembl 110, assembly GRCh38.p14
RNA pseudouridine synthase D4 [Source:HGNC Symbol;Acc:HGNC:25898]
E-value: 3.5e-14
Percent Identity: 30.1

References

A pseudouridine synthase required for the formation of two universally conserved pseudouridines in ribosomal RNA is essential for normal growth of Escherichia coli.
Raychaudhuri S, Conrad J, Hall BG, Ofengand J
RNA 1998 Nov;4(11):1407-17
PubMed ID: 9814761