Pseudomonas aeruginosa PAO1, PA4552 (pilW)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA4552
Name
pilW
Replicon chromosome
Genomic location 5099263 - 5100087 (+ strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs
CommentAlgR positively regulates the fimU-pilVWXY1Y2E operon

Cross-References

RefSeq NP_253242.1
GI 15599748
Affymetrix PA4552_pilW_at
Entrez 879456
GenBank AAG07940.1
INSDC AAG07940.1
NCBI Locus Tag PA4552
protein_id(GenBank) gb|AAG07940.1|AE004869_4|gnl|PseudoCAP|PA4552
TIGR NTL03PA04553
UniParc UPI00000D4242
UniProtKB Acc G3XD15
UniProtKB ID G3XD15_PSEAE
UniRef100 UniRef100_W0YXV9
UniRef50 UniRef50_W0YXV9
UniRef90 UniRef90_W0YXV9

Product

Feature Type CDS
Coding Frame 1
Product Name
type 4 fimbrial biogenesis protein PilW
Product Name Confidence: Class 1
Synonyms
Evidence for Translation
Charge (pH 7) 3.87
Kyte-Doolittle Hydrophobicity Value -0.310
Molecular Weight (kDa) 30.1
Isoelectric Point (pI) 8.83

Subcellular localization

Individual Mappings
Localization Confidence PMID
Unknown Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Virulence Evidence

Source 1
Database VFDB : VF0082
Category Type IVa pili
Evidence
Host Organism
Notes
Reference

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 22 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG004212 (350 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA4552
Search term: pilW

Human Homologs

References

Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res 2005 Feb;15(2):321-9
PubMed ID: 15687295
Identification of a gene, pilV, required for type 4 fimbrial biogenesis in Pseudomonas aeruginosa, whose product possesses a pre-pilin-like leader sequence.
Alm RA, Mattick JS
Mol Microbiol 1995 May;16(3):485-96
PubMed ID: 7565109
Fimbrial biogenesis genes of Pseudomonas aeruginosa: pilW and pilX increase the similarity of type 4 fimbriae to the GSP protein-secretion systems and pilY1 encodes a gonococcal PilC homologue.
Alm RA, Hallinan JP, Watson AA, Mattick JS
Mol Microbiol 1996 Oct;22(1):161-73
PubMed ID: 8899718
Genes involved in the biogenesis and function of type-4 fimbriae in Pseudomonas aeruginosa.
Alm RA, Mattick JS
Gene 1997 Jun 11;192(1):89-98
PubMed ID: 9224878
Pseudomonas aeruginosa AlgR regulates type IV pilus biosynthesis by activating transcription of the fimU-pilVWXY1Y2E operon.
Belete B, Lu H, Wozniak DJ
J Bacteriol 2008 Mar;190(6):2023-30
PubMed ID: 18178737