Pseudomonas aeruginosa PAO1, PA4553 (pilX)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA4553
Name
pilX
Replicon chromosome
Genomic location 5100084 - 5100671 (+ strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs
CommentAlgR positively regulates the fimU-pilVWXY1Y2E operon

Cross-References

RefSeq NP_253243.1
GI 15599749
Affymetrix PA4553_pilX_at
Entrez 877858
GenBank AAG07941.1
INSDC AAG07941.1
NCBI Locus Tag PA4553
protein_id(GenBank) gb|AAG07941.1|AE004869_5|gnl|PseudoCAP|PA4553
TIGR NTL03PA04554
UniParc UPI00000C5D5F
UniProtKB Acc Q9HVM9
UniProtKB ID Q9HVM9_PSEAE
UniRef100 UniRef100_Q9HVM9
UniRef50 UniRef50_Q9HVM9
UniRef90 UniRef90_Q9HVM9

Product

Feature Type CDS
Coding Frame 1
Product Name
type 4 fimbrial biogenesis protein PilX
Product Name Confidence: Class 1
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -0.83
Kyte-Doolittle Hydrophobicity Value -0.127
Molecular Weight (kDa) 21.1
Isoelectric Point (pI) 5.92

Subcellular localization

Individual Mappings
Localization Confidence PMID
Unknown Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Virulence Evidence

Source 1
Database VFDB : VF0082
Category Type IVa pili
Evidence
Host Organism
Notes
Reference

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 6 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG004213 (394 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA4553
Search term: pilX

Human Homologs

References

Identification of a gene, pilV, required for type 4 fimbrial biogenesis in Pseudomonas aeruginosa, whose product possesses a pre-pilin-like leader sequence.
Alm RA, Mattick JS
Mol Microbiol 1995 May;16(3):485-96
PubMed ID: 7565109
Pseudomonas aeruginosa AlgR regulates type IV pilus biosynthesis by activating transcription of the fimU-pilVWXY1Y2E operon.
Belete B, Lu H, Wozniak DJ
J Bacteriol 2008 Mar;190(6):2023-30
PubMed ID: 18178737