Pseudomonas aeruginosa PAO1, PA4559 (lspA)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA4559
Name
lspA
Replicon chromosome
Genomic location 5106449 - 5106958 (- strand)

Cross-References

RefSeq NP_253249.1
GI 15599755
Affymetrix PA4559_lspA_at
DNASU PaCD00007974
Entrez 877620
GenBank AAG07947.1
INSDC AAG07947.1
NCBI Locus Tag PA4559
protein_id(GenBank) gb|AAG07947.1|AE004869_11|gnl|PseudoCAP|PA4559
TIGR NTL03PA04560
UniParc UPI00000D42A0
UniProtKB Acc Q9HVM5
UniProtKB ID LSPA_PSEAE
UniRef100 UniRef100_Q9HVM5
UniRef50 UniRef50_Q02GB8
UniRef90 UniRef90_Q02GB8

Product

Feature Type CDS
Coding Frame 1
Product Name
prolipoprotein signal peptidase
Product Name Confidence: Class 2
Synonyms
Evidence for Translation
Charge (pH 7) 1.70
Kyte-Doolittle Hydrophobicity Value 0.615
Molecular Weight (kDa) 19.0
Isoelectric Point (pI) 8.93

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Membrane Class 2
Cytoplasmic Membrane Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
6FMS HYDROLASE 02/02/18 IMISX-EP of Se-LspA Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1); SYNTHETIC CONSTRUCT 3 X-RAY DIFFRACTION 100.0
5DIR HYDROLASE 09/01/15 membrane protein at 2.8 Angstroms Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228); SYNTHETIC CONSTRUCT 2.8 X-RAY DIFFRACTION 100.0

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 510 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG004218 (536 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA4559
Search term: lspA

Human Homologs

References

Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res 2005 Feb;15(2):321-9
PubMed ID: 15687295
Nucleotide sequence of the Pseudomonas fluorescens signal peptidase II gene (lsp) and flanking genes.
Isaki L, Beers R, Wu HC
J Bacteriol 1990 Nov;172(11):6512-7
PubMed ID: 2121716