Pseudomonas aeruginosa PAO1, PA4563 (rpsT)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA4563
Name
rpsT
Replicon chromosome
Genomic location 5112663 - 5112938 (+ strand)

Cross-References

RefSeq NP_253253.1
GI 15599759
Affymetrix PA4563_rpsT_at
DNASU PaCD00007976
Entrez 879605
GenBank AAG07951.1
INSDC AAG07951.1
NCBI Locus Tag PA4563
protein_id(GenBank) gb|AAG07951.1|AE004870_2|gnl|PseudoCAP|PA4563
TIGR NTL03PA04564
UniParc UPI0000134E51
UniProtKB Acc Q9HVM1
UniProtKB ID RS20_PSEAE
UniRef100 UniRef100_B7V0A6
UniRef50 UniRef50_Q2KZD7
UniRef90 UniRef90_B7V0A6

Product

Feature Type CDS
Coding Frame 1
Product Name
30S ribosomal protein S20
Product Name Confidence: Class 2
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) 15.94
Kyte-Doolittle Hydrophobicity Value -0.516
Molecular Weight (kDa) 9.9
Isoelectric Point (pI) 11.77

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Cytoplasmic Class 1
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
6SPG RIBOSOME 09/01/19 Pseudomonas aeruginosa 70s ribosome from a clinical isolate Pseudomonas aeruginosa 3.34 ELECTRON MICROSCOPY 100.0
6SPF RIBOSOME 09/01/19 Pseudomonas aeruginosa 70s ribosome from an aminoglycoside resistant clinical isolate Pseudomonas aeruginosa 2.89 ELECTRON MICROSCOPY 100.0
7UNV RIBOSOME 04/11/22 Pseudomonas aeruginosa 70S ribosome initiation complex bound to IF2-GDPCP (structure II-A) Escherichia coli; Pseudomonas aeruginosa PAO1; SYNTHETIC CONSTRUCT 2.7 ELECTRON MICROSCOPY 100.0
6SPC RIBOSOME 09/01/19 Pseudomonas aeruginosa 30s ribosome from an aminoglycoside resistant clinical isolate Pseudomonas aeruginosa 2.95 ELECTRON MICROSCOPY 100.0
7UNR RIBOSOME 04/11/22 Pseudomonas aeruginosa 70S ribosome initiation complex bound to compact IF2-GDP (composite structure I-A) Escherichia coli; Pseudomonas aeruginosa PAO1; SYNTHETIC CONSTRUCT 2.9 ELECTRON MICROSCOPY 100.0
7UNW RIBOSOME 04/11/22 Pseudomonas aeruginosa 70S ribosome initiation complex bound to IF2-GDPCP (structure II-B) Escherichia coli; Pseudomonas aeruginosa PAO1; SYNTHETIC CONSTRUCT 2.6 ELECTRON MICROSCOPY 100.0
7UNU RIBOSOME 04/11/22 Pseudomonas aeruginosa 70S ribosome initiation complex bound to compact IF2-GDP (composite structure I-B) Escherichia coli; Pseudomonas aeruginosa PAO1; SYNTHETIC CONSTRUCT 2.9 ELECTRON MICROSCOPY 100.0
6SPE RIBOSOME 09/01/19 Pseudomonas aeruginosa 30s ribosome from a clinical isolate Pseudomonas aeruginosa 3.6 ELECTRON MICROSCOPY 100.0

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 549 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG004222 (535 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA4563
Search term: rpsT

Human Homologs

References

Primary structure of protein S20 from the small ribosomal subunit of Escherichia coli.
Wittmann-Liebold B, Marzinzig E, Lehmann A
FEBS Lett 1976 Sep 15;68(1):110-4
PubMed ID: 786731
Structure of the DNA distal to the gene for ribosomal protein S20 in Escherichia coli K12: presence of a strong terminator and an IS1 element.
Mackie GA
Nucleic Acids Res 1986 Sep 11;14(17):6965-81
PubMed ID: 2429258
Ribosome activity and modification of 16S RNA are influenced by deletion of ribosomal protein S20.
Rydén-Aulin M, Shaoping Z, Kylsten P, Isaksson LA
Mol Microbiol 1993 Mar;7(6):983-92
PubMed ID: 7683367
Relationship of the expression of the S20 and L34 ribosomal proteins to polyamine biosynthesis in Escherichia coli.
Panagiotidis CA, Huang SC, Canellakis ES
Int J Biochem Cell Biol 1995 Feb;27(2):157-68
PubMed ID: 7539334