Pseudomonas aeruginosa PAO1, PA4567 (rpmA)

Gene Feature Overview

Strain	Pseudomonas aeruginosa PAO1 (Stover et al., 2000) GCF_000006765.1|latest
Locus Tag	PA4567
Name	rpmA
Replicon	chromosome
Genomic location	5116032 - 5116289 (- strand)

Cross-References

RefSeq	NP_253257.1
GI	15599763
Affymetrix	PA4567_rpmA_at
DNASU	PaCD00007977
Entrez	880878
GenBank	AAG07955.1
INSDC	AAG07955.1
NCBI Locus Tag	PA4567
protein_id(GenBank)	gb|AAG07955.1|AE004870_6|gnl|PseudoCAP|PA4567
TIGR	NTL03PA04568
UniParc	UPI0000133E10
UniProtKB Acc	Q9HVL7
UniProtKB ID	RL27_PSEAE
UniRef100	UniRef100_A6VBV4
UniRef50	UniRef50_P44363
UniRef90	UniRef90_A6VBV4

Product

Feature Type	CDS
Coding Frame	1
Product Name	50S ribosomal protein L27 Product Name Confidence: Class 2
Synonyms
Evidence for Translation	Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7)	12.70
Kyte-Doolittle Hydrophobicity Value	-0.326
Molecular Weight (kDa)	9.0
Isoelectric Point (pI)	11.37

Subcellular localization

Individual Mappings	Localization Confidence PMID Cytoplasmic Class 1 Cytoplasmic Class 3 20472543
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession	Header	Accession Date	Compound	Source	Resolution	Method	Percent Identity
6SPD	RIBOSOME	09/01/19	Pseudomonas aeruginosa 50s ribosome from a clinical isolate	Pseudomonas aeruginosa	3.28	ELECTRON MICROSCOPY	98.7
7UNV	RIBOSOME	04/11/22	Pseudomonas aeruginosa 70S ribosome initiation complex bound to IF2-GDPCP (structure II-A)	Escherichia coli; Pseudomonas aeruginosa PAO1; SYNTHETIC CONSTRUCT	2.7	ELECTRON MICROSCOPY	100.0
6SPG	RIBOSOME	09/01/19	Pseudomonas aeruginosa 70s ribosome from a clinical isolate	Pseudomonas aeruginosa	3.34	ELECTRON MICROSCOPY	98.7
7UNR	RIBOSOME	04/11/22	Pseudomonas aeruginosa 70S ribosome initiation complex bound to compact IF2-GDP (composite structure I-A)	Escherichia coli; Pseudomonas aeruginosa PAO1; SYNTHETIC CONSTRUCT	2.9	ELECTRON MICROSCOPY	100.0
6SPB	RIBOSOME	09/01/19	Pseudomonas aeruginosa 50s ribosome from a clinical isolate with a mutation in uL6	Pseudomonas aeruginosa	2.82	ELECTRON MICROSCOPY	98.7
7UNU	RIBOSOME	04/11/22	Pseudomonas aeruginosa 70S ribosome initiation complex bound to compact IF2-GDP (composite structure I-B)	Escherichia coli; Pseudomonas aeruginosa PAO1; SYNTHETIC CONSTRUCT	2.9	ELECTRON MICROSCOPY	100.0
6SPF	RIBOSOME	09/01/19	Pseudomonas aeruginosa 70s ribosome from an aminoglycoside resistant clinical isolate	Pseudomonas aeruginosa	2.89	ELECTRON MICROSCOPY	98.7
7UNW	RIBOSOME	04/11/22	Pseudomonas aeruginosa 70S ribosome initiation complex bound to IF2-GDPCP (structure II-B)	Escherichia coli; Pseudomonas aeruginosa PAO1; SYNTHETIC CONSTRUCT	2.6	ELECTRON MICROSCOPY	100.0

Pathogen Association Analysis

Results

Common Found in both pathogen and nonpathogenic strains Hits to this gene were found in 592 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database	View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group	POG004226 (535 members)
Putative Inparalogs	None Found

Interactions

STRING database

Search for predicted protein-protein interactions using: Search term: PA4567 Search term: rpmA Search term: 50S ribosomal protein L27

Human Homologs

References

Cloning and nucleotide sequencing of the genes, rpIU and rpmA, for ribosomal proteins L21 and L27 of Escherichia coli. Jeong JH, Kitakawa M, Isono S, Isono K DNA Seq 1993;4(1):59-67 PubMed ID: 8312607

Ribosomal protein L27 participates in both 50 S subunit assembly and the peptidyl transferase reaction. Wower IK, Wower J, Zimmermann RA J Biol Chem 1998 Jul 31;273(31):19847-52 PubMed ID: 9677420