Pseudomonas aeruginosa PAO1, PA4568 (rplU)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA4568
Name
rplU
Replicon chromosome
Genomic location 5116313 - 5116624 (- strand)
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_253258.1
GI 15599764
Affymetrix PA4568_rplU_at
DNASU PaCD00007978
Entrez 881148
GenBank AAG07956.1
INSDC AAG07956.1
NCBI Locus Tag PA4568
protein_id(GenBank) gb|AAG07956.1|AE004870_7|gnl|PseudoCAP|PA4568
TIGR NTL03PA04569
UniParc UPI00000C5D67
UniProtKB Acc Q9HVL6
UniProtKB ID RL21_PSEAE
UniRef100 UniRef100_B7V0B1
UniRef50 UniRef50_B7V0B1
UniRef90 UniRef90_B7V0B1

Product

Feature Type CDS
Coding Frame 1
Product Name
50S ribosomal protein L21
Product Name Confidence: Class 2
Synonyms
Evidence for Translation
Detected using LC-MS/MS (11.65 kDa, PMID:21751344)
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) 7.43
Kyte-Doolittle Hydrophobicity Value -0.316
Molecular Weight (kDa) 11.7
Isoelectric Point (pI) 10.58

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Outer Membrane Vesicle Class 1
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
6SPG RIBOSOME 09/01/19 Pseudomonas aeruginosa 70s ribosome from a clinical isolate Pseudomonas aeruginosa 3.34 ELECTRON MICROSCOPY 100.0
6SPF RIBOSOME 09/01/19 Pseudomonas aeruginosa 70s ribosome from an aminoglycoside resistant clinical isolate Pseudomonas aeruginosa 2.89 ELECTRON MICROSCOPY 100.0
7UNV RIBOSOME 04/11/22 Pseudomonas aeruginosa 70S ribosome initiation complex bound to IF2-GDPCP (structure II-A) Escherichia coli; Pseudomonas aeruginosa PAO1; SYNTHETIC CONSTRUCT 2.7 ELECTRON MICROSCOPY 100.0
7UNW RIBOSOME 04/11/22 Pseudomonas aeruginosa 70S ribosome initiation complex bound to IF2-GDPCP (structure II-B) Escherichia coli; Pseudomonas aeruginosa PAO1; SYNTHETIC CONSTRUCT 2.6 ELECTRON MICROSCOPY 100.0
6SPB RIBOSOME 09/01/19 Pseudomonas aeruginosa 50s ribosome from a clinical isolate with a mutation in uL6 Pseudomonas aeruginosa 2.82 ELECTRON MICROSCOPY 100.0
7UNU RIBOSOME 04/11/22 Pseudomonas aeruginosa 70S ribosome initiation complex bound to compact IF2-GDP (composite structure I-B) Escherichia coli; Pseudomonas aeruginosa PAO1; SYNTHETIC CONSTRUCT 2.9 ELECTRON MICROSCOPY 100.0
7UNR RIBOSOME 04/11/22 Pseudomonas aeruginosa 70S ribosome initiation complex bound to compact IF2-GDP (composite structure I-A) Escherichia coli; Pseudomonas aeruginosa PAO1; SYNTHETIC CONSTRUCT 2.9 ELECTRON MICROSCOPY 100.0
6SPD RIBOSOME 09/01/19 Pseudomonas aeruginosa 50s ribosome from a clinical isolate Pseudomonas aeruginosa 3.28 ELECTRON MICROSCOPY 100.0

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 590 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG004227 (534 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA4568
Search term: rplU

Human Homologs

References

Amino acid sequence of the ribosomal protein L21 of Escherichia coli.
Heiland I, Wittmann-Liebold B
Biochemistry 1979 Oct 16;18(21):4605-12
PubMed ID: 387076
Precise determination of RNA-protein contact sites in the 50 S ribosomal subunit of Escherichia coli.
Thiede B, Urlaub H, Neubauer H, Grelle G, Wittmann-Liebold B
Biochem J 1998 Aug 15;334 ( Pt 1)(Pt 1):39-42
PubMed ID: 9693099
Proteomic analysis of outer membrane vesicles derived from Pseudomonas aeruginosa.
Choi DS, Kim DK, Choi SJ, Lee J, Choi JP, Rho S, Park SH, Kim YK, Hwang D, Gho YS
Proteomics 2011 Aug;11(16):3424-9
PubMed ID: 21751344