Pseudomonas aeruginosa PAO1, PA4597 (oprJ)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA4597
Name
oprJ
Replicon chromosome
Genomic location 5149633 - 5151072 (- strand)
Transposon Mutants 3 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_253287.1
GI 15599793
Affymetrix PA4597_oprJ_at
Entrez 881070
GenBank AAG07985.1
INSDC AAG07985.1
NCBI Locus Tag PA4597
protein_id(GenBank) gb|AAG07985.1|AE004873_7|gnl|PseudoCAP|PA4597
TIGR NTL03PA04598
UniParc UPI0000130D97
UniProtKB Acc Q51397
UniProtKB ID OPRJ_PSEAE
UniRef100 UniRef100_Q51397
UniRef50 UniRef50_Q51397
UniRef90 UniRef90_Q51397

Product

Feature Type CDS
Coding Frame 1
Product Name
Multidrug efflux outer membrane protein OprJ precursor
Product Name Confidence: Class 1
Synonyms
Evidence for Translation
Charge (pH 7) 4.71
Kyte-Doolittle Hydrophobicity Value -0.252
Molecular Weight (kDa) 52.0
Isoelectric Point (pI) 9.38

Subcellular localization

Individual Mappings
Localization Confidence PMID
Outer Membrane Class 3
Outer Membrane Class 1
Additional evidence for subcellular localization

AMR gene predictions from CARD database

Identified using the Resistance Gene Identifier (RGI)

Model Name Definition Accession Model Type (?) SNP Cutoff Bit Score Percent Identity CARD Version
OprJ OprJ is the outer membrane channel component of the MexCD-OprJ multidrug efflux complex. [PMID:9449262] ARO:3000802 protein homolog model Strict 882.1 99.58 3.2.5

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
5AZS MEMBRANE PROTEIN 10/21/15 Crystal structure of a membrane protein from Pseudomonas aeruginosa Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228) 3.1 X-RAY DIFFRACTION 100.0

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 293 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG004255 (330 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA4597
Search term: oprJ

Human Homologs

References

Overexpression of the mexC-mexD-oprJ efflux operon in nfxB-type multidrug-resistant strains of Pseudomonas aeruginosa.
Poole K, Gotoh N, Tsujimoto H, Zhao Q, Wada A, Yamasaki T, Neshat S, Yamagishi J, Li XZ, Nishino T
Mol Microbiol 1996 Aug;21(4):713-24
PubMed ID: 8878035