Pseudomonas aeruginosa PAO1, PA4598 (mexD)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA4598
Name
mexD
Replicon chromosome
Genomic location 5151078 - 5154209 (- strand)
Transposon Mutants 4 transposon mutants in PAO1
Transposon Mutants in orthologs 8 transposon mutants in orthologs

Cross-References

RefSeq NP_253288.1
GI 15599794
Affymetrix PA4598_mexD_at
Entrez 881071
GenBank AAG07986.1
INSDC AAG07986.1
NCBI Locus Tag PA4598
protein_id(GenBank) gb|AAG07986.1|AE004873_8|gnl|PseudoCAP|PA4598
TIGR NTL03PA04599
UniParc UPI00000C5D80
UniProtKB Acc Q9HVI9
UniProtKB ID Q9HVI9_PSEAE
UniRef100 UniRef100_Q9HVI9
UniRef50 UniRef50_P31224
UniRef90 UniRef90_Q9HVI9

Product

Feature Type CDS
Coding Frame 1
Product Name
Resistance-Nodulation-Cell Division (RND) multidrug efflux transporter MexD
Product Name Confidence: Class 1
Synonyms RND multidrug efflux transporter MexD
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -16.84
Kyte-Doolittle Hydrophobicity Value 0.401
Molecular Weight (kDa) 111.6
Isoelectric Point (pI) 4.83

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Membrane Class 3
Cytoplasmic Membrane Class 2
Additional evidence for subcellular localization

AMR gene predictions from CARD database

Identified using the Resistance Gene Identifier (RGI)

Model Name Definition Accession Model Type (?) SNP Cutoff Bit Score Percent Identity CARD Version
MexD MexD is the multidrug inner membrane transporter of the MexCD-OprJ complex. [PMID:9449262, PMID:8878035] ARO:3000801 protein homolog model Strict 1845.1 95.62 3.2.5

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 487 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000417 (2093 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA4598
Search term: mexD

Human Homologs

References

Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res 2005 Feb;15(2):321-9
PubMed ID: 15687295
Overexpression of the mexC-mexD-oprJ efflux operon in nfxB-type multidrug-resistant strains of Pseudomonas aeruginosa.
Poole K, Gotoh N, Tsujimoto H, Zhao Q, Wada A, Yamasaki T, Neshat S, Yamagishi J, Li XZ, Nishino T
Mol Microbiol 1996 Aug;21(4):713-24
PubMed ID: 8878035