Pseudomonas aeruginosa PAO1, PA4649 (cupE2)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA4649
Name
cupE2
Replicon chromosome
Genomic location 5215670 - 5216203 (+ strand)
Transposon Mutants 3 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs
CommentThe cupE gene cluster encodes a chaperone usher pathway that is homologous to the A. baumanii csu gene cluster. Activation of the cupE genes leads to the production and assembly of CupE fimbriae on the cell surface. These fimbriae are important in early and late stages of biofilm formation. The expression of the cupE gene cluster is controlled by the two-component system PprA-PprB, and binding of PprB to the cupE1 promoter has been confirmed by EMSA.

Cross-References

RefSeq NP_253338.1
GI 15599844
Affymetrix PA4649_at
DNASU PaCD00008016
Entrez 881286
GenBank AAG08036.1
INSDC AAG08036.1
NCBI Locus Tag PA4649
protein_id(GenBank) gb|AAG08036.1|AE004879_2|gnl|PseudoCAP|PA4649
TIGR NTL03PA04650
UniParc UPI00000C5DAA
UniProtKB Acc Q9HVE3
UniProtKB ID Q9HVE3_PSEAE
UniRef100 UniRef100_W0YYA0
UniRef50 UniRef50_H8NWE8
UniRef90 UniRef90_W0YYA0

Product

Feature Type CDS
Coding Frame 1
Product Name
Pilin subunit CupE2
Product Name Confidence: Class 1
Synonyms
Evidence for Translation
Charge (pH 7) -0.15
Kyte-Doolittle Hydrophobicity Value 0.190
Molecular Weight (kDa) 18.2
Isoelectric Point (pI) 6.32

Subcellular localization

Individual Mappings
Localization Confidence PMID
Extracellular Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 48 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG004302 (309 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA4649
Search term: cupE2

Human Homologs

References

Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res 2005 Feb;15(2):321-9
PubMed ID: 15687295
The PprA-PprB two-component system activates CupE, the first non-archetypal Pseudomonas aeruginosa chaperone-usher pathway system assembling fimbriae.
Giraud C, Bernard CS, Calderon V, Yang L, Filloux A, Molin S, Fichant G, Bordi C, de Bentzmann S
Environ Microbiol 2011 Mar;13(3):666-83
PubMed ID: 21091863