Pseudomonas aeruginosa PAO1, PA4687 (hitA)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA4687
Name
hitA
Replicon chromosome
Genomic location 5257696 - 5258703 (+ strand)
Transposon Mutants 5 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_253376.1
GI 15599882
Affymetrix PA4687_hitA_at
DNASU PaCD00008037
Entrez 881454
GenBank AAG08074.1
INSDC AAG08074.1
NCBI Locus Tag PA4687
protein_id(GenBank) gb|AAG08074.1|AE004882_5|gnl|PseudoCAP|PA4687
TIGR NTL03PA04688
UniParc UPI00000C5DC7
UniProtKB Acc Q9HVA8
UniProtKB ID Q9HVA8_PSEAE
UniRef100 UniRef100_W0YXB5
UniRef50 UniRef50_V6JBE1
UniRef90 UniRef90_A6VCD9

Product

Feature Type CDS
Coding Frame 1
Product Name
ferric iron-binding periplasmic protein HitA
Product Name Confidence: Class 1
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Detected by MALDI-TOF/TOF (PMID:19333994).
Charge (pH 7) -4.82
Kyte-Doolittle Hydrophobicity Value -0.223
Molecular Weight (kDa) 36.1
Isoelectric Point (pI) 5.48

Subcellular localization

Individual Mappings
Localization Confidence PMID
Periplasmic Class 3
Extracellular Class 1
mass spectrometry evidence used in manual assertion
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
6IVY STRUCTURAL PROTEIN 12/04/18 Crystal structure of iron-bound HitA from Pseudomonas aeruginosa Pseudomonas aeruginosa PAO1 2 X-RAY DIFFRACTION 100.0
6IWF METAL BINDING PROTEIN 12/05/18 Crystal structure of HitA from Pseudomonas aeruginosa Pseudomonas aeruginosa PAO1 1.70662 X-RAY DIFFRACTION 100.0
6J2S METAL BINDING PROTEIN 01/02/19 Structure of HitA bound to gallium from Pseudomonas aeruginosa Pseudomonas aeruginosa 1.73005 X-RAY DIFFRACTION 100.0

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 314 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG004337 (456 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA4687
Search term: hitA

Human Homologs

References

Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res 2005 Feb;15(2):321-9
PubMed ID: 15687295
Identification of two iron-repressed periplasmic proteins in Haemophilus influenzae.
Harkness RE, Chong P, Klein MH
J Bacteriol 1992 Apr;174(8):2425-30
PubMed ID: 1556062
Structure of Haemophilus influenzae Fe(+3)-binding protein reveals convergent evolution within a superfamily.
Bruns CM, Nowalk AJ, Arvai AS, McTigue MA, Vaughan KG, Mietzner TA, McRee DE
Nat Struct Biol 1997 Nov;4(11):919-24
PubMed ID: 9360608
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994
Mep72, a metzincin protease that is preferentially secreted by biofilms of Pseudomonas aeruginosa.
Passmore IJ, Nishikawa K, Lilley KS, Bowden SD, Chung JC, Welch M
J Bacteriol 2015 Feb 15;197(4):762-73
PubMed ID: 25488299
Isolation and characterization of gallium resistant Pseudomonas aeruginosa mutants.
García-Contreras R, Lira-Silva E, Jasso-Chávez R, Hernández-González IL, Maeda T, Hashimoto T, Boogerd FC, Sheng L, Wood TK, Moreno-Sánchez R
Int J Med Microbiol 2013 Dec;303(8):574-82
PubMed ID: 23992635