Strain |
Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest |
Locus Tag |
PA4687
|
Name |
hitA
|
Replicon | chromosome |
Genomic location | 5257696 - 5258703 (+ strand) |
Transposon Mutants | 5 transposon mutants in PAO1 |
Transposon Mutants in orthologs | 1 transposon mutants in orthologs |
RefSeq | NP_253376.1 |
GI | 15599882 |
Affymetrix | PA4687_hitA_at |
DNASU | PaCD00008037 |
Entrez | 881454 |
GenBank | AAG08074.1 |
INSDC | AAG08074.1 |
NCBI Locus Tag | PA4687 |
protein_id(GenBank) | gb|AAG08074.1|AE004882_5|gnl|PseudoCAP|PA4687 |
TIGR | NTL03PA04688 |
UniParc | UPI00000C5DC7 |
UniProtKB Acc | Q9HVA8 |
UniProtKB ID | Q9HVA8_PSEAE |
UniRef100 | UniRef100_W0YXB5 |
UniRef50 | UniRef50_V6JBE1 |
UniRef90 | UniRef90_A6VCD9 |
Feature Type | CDS |
Coding Frame | 1 |
Product Name |
ferric iron-binding periplasmic protein HitA
|
Synonyms | |
Evidence for Translation |
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Detected by MALDI-TOF/TOF (PMID:19333994).
|
Charge (pH 7) | -4.82 |
Kyte-Doolittle Hydrophobicity Value | -0.223 |
Molecular Weight (kDa) | 36.1 |
Isoelectric Point (pI) | 5.48 |
Individual Mappings | |
Additional evidence for subcellular localization |
Accession | Header | Accession Date | Compound | Source | Resolution | Method | Percent Identity |
6IVY | STRUCTURAL PROTEIN | 12/04/18 | Crystal structure of iron-bound HitA from Pseudomonas aeruginosa | Pseudomonas aeruginosa PAO1 | 2 | X-RAY DIFFRACTION | 100.0 |
6IWF | METAL BINDING PROTEIN | 12/05/18 | Crystal structure of HitA from Pseudomonas aeruginosa | Pseudomonas aeruginosa PAO1 | 1.70662 | X-RAY DIFFRACTION | 100.0 |
6J2S | METAL BINDING PROTEIN | 01/02/19 | Structure of HitA bound to gallium from Pseudomonas aeruginosa | Pseudomonas aeruginosa | 1.73005 | X-RAY DIFFRACTION | 100.0 |
Results |
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 314 genera
|
Pseudomonas Ortholog Database | View orthologs at Pseudomonas Ortholog Database |
Pseudomonas Ortholog Group |
POG004337 (456 members) |
Putative Inparalogs | None Found |
STRING database | Search for predicted protein-protein interactions using:
Search term: PA4687
Search term: hitA
Search term: ferric iron-binding periplasmic protein HitA
|
Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res 2005 Feb;15(2):321-9
PubMed ID: 15687295
|
Identification of two iron-repressed periplasmic proteins in Haemophilus influenzae.
Harkness RE, Chong P, Klein MH
J Bacteriol 1992 Apr;174(8):2425-30
PubMed ID: 1556062
|
Structure of Haemophilus influenzae Fe(+3)-binding protein reveals convergent evolution within a superfamily.
Bruns CM, Nowalk AJ, Arvai AS, McTigue MA, Vaughan KG, Mietzner TA, McRee DE
Nat Struct Biol 1997 Nov;4(11):919-24
PubMed ID: 9360608
|
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994
|
Mep72, a metzincin protease that is preferentially secreted by biofilms of Pseudomonas aeruginosa.
Passmore IJ, Nishikawa K, Lilley KS, Bowden SD, Chung JC, Welch M
J Bacteriol 2015 Feb 15;197(4):762-73
PubMed ID: 25488299
|
Isolation and characterization of gallium resistant Pseudomonas aeruginosa mutants.
García-Contreras R, Lira-Silva E, Jasso-Chávez R, Hernández-González IL, Maeda T, Hashimoto T, Boogerd FC, Sheng L, Wood TK, Moreno-Sánchez R
Int J Med Microbiol 2013 Dec;303(8):574-82
PubMed ID: 23992635
|