Pseudomonas aeruginosa PAO1, PA4700 (mrcB)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA4700
Name
mrcB
Synonym: pbpF
Synonym: ponB
Replicon chromosome
Genomic location 5277968 - 5280292 (- strand)
Transposon Mutants 7 transposon mutants in PAO1
Transposon Mutants in orthologs 3 transposon mutants in orthologs

Cross-References

RefSeq NP_253388.1
GI 15599894
Affymetrix PA4700_mrcB_at
Entrez 881511
GenBank AAG08086.1
INSDC AAG08086.1
NCBI Locus Tag PA4700
protein_id(GenBank) gb|AAG08086.1|AE004884_4|gnl|PseudoCAP|PA4700
TIGR NTL03PA04701
UniParc UPI00000D424A
UniProtKB Acc G3XD31
UniProtKB ID G3XD31_PSEAE
UniRef100 UniRef100_G3XD31
UniRef50 UniRef50_Q4K603
UniRef90 UniRef90_A6VCF4

Product

Feature Type CDS
Coding Frame 1
Product Name
penicillin-binding protein 1B
Product Name Confidence: Class 2
Synonyms peptidoglycan synthetase
Evidence for Translation
Detected using LC-MS/MS (85.49 kDa, PMID:21751344)
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) 11.89
Kyte-Doolittle Hydrophobicity Value -0.282
Molecular Weight (kDa) 85.5
Isoelectric Point (pI) 9.57

Subcellular localization

Individual Mappings
Localization Confidence PMID
Outer Membrane Vesicle Class 1
Cytoplasmic Membrane Class 3
Cytoplasmic Membrane Class 2
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Drugs Targeting this Protein

Identified by Diamond using e-value cutoff of 0.0001 and returning alignments that span 100% of the query sequence and that have more than 95% identity.

Drug Name Source Accession Source DB Version Target Accession Target Description Percent Identity Alignment Length E-Value
Cefoperazone DB01329 DrugBank 5.1.4 Q9X6W0 Penicillin-binding protein 1B 100.0 774 0.0e+00
Ceftolozane DB09050 DrugBank 5.1.4 Q9X6W0 Penicillin-binding protein 1B 100.0 774 0.0e+00
Ceftazidime DB00438 DrugBank 5.1.4 Q9X6W0 Penicillin-binding protein 1B 100.0 774 0.0e+00
Cefpiramide DB00430 DrugBank 5.1.4 Q9X6W0 Penicillin-binding protein 1B 100.0 774 0.0e+00

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 553 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG004349 (535 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA4700
Search term: mrcB

Human Homologs

References

Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res 2005 Feb;15(2):321-9
PubMed ID: 15687295
In vitro peptidoglycan polymerization catalysed by penicillin binding protein 1b of Escherichia coli K-12.
Suzuki H, van Heijenoort Y, Tamura T, Mizoguchi J, Hirota Y, van Heijenoort J
FEBS Lett 1980 Feb 11;110(2):245-9
PubMed ID: 6989636
Molecular divergence of a major peptidoglycan synthetase with transglycosylase-transpeptidase activities in Escherichia coli --- penicillin-binding protein 1Bs.
Nakagawa J, Matsuhashi M
Biochem Biophys Res Commun 1982 Apr 29;105(4):1546-53
PubMed ID: 7049165
Functional biosynthesis of cell wall peptidoglycan by polymorphic bifunctional polypeptides. Penicillin-binding protein 1Bs of Escherichia coli with activities of transglycosylase and transpeptidase.
Nakagawa J, Tamaki S, Tomioka S, Matsuhashi M
J Biol Chem 1984 Nov 25;259(22):13937-46
PubMed ID: 6389538
Purification and sequencing of the active site tryptic peptide from penicillin-binding protein 1b of Escherichia coli.
Nicholas RA, Suzuki H, Hirota Y, Strominger JL
Biochemistry 1985 Jul 2;24(14):3448-53
PubMed ID: 3899167
Topographical and functional investigation of Escherichia coli penicillin-binding protein 1b by alanine stretch scanning mutagenesis.
Lefèvre F, Rémy MH, Masson JM
J Bacteriol 1997 Aug;179(15):4761-7
PubMed ID: 9244263
Proteomic analysis of outer membrane vesicles derived from Pseudomonas aeruginosa.
Choi DS, Kim DK, Choi SJ, Lee J, Choi JP, Rho S, Park SH, Kim YK, Hwang D, Gho YS
Proteomics 2011 Aug;11(16):3424-9
PubMed ID: 21751344