Pseudomonas aeruginosa PAO1, PA4733 (acsB)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA4733
Name
acsB
Replicon chromosome
Genomic location 5315534 - 5317471 (+ strand)
Transposon Mutants 1 transposon mutants in PAO1
Transposon Mutants in orthologs 3 transposon mutants in orthologs

Cross-References

RefSeq NP_253421.1
GI 15599927
Affymetrix PA4733_acsB_at
Entrez 881650
GenBank AAG08119.1
INSDC AAG08119.1
NCBI Locus Tag PA4733
protein_id(GenBank) gb|AAG08119.1|AE004887_3|gnl|PseudoCAP|PA4733
TIGR NTL03PA04734
UniParc UPI00000C5DE5
UniProtKB Acc Q9HV66
UniProtKB ID ACSA2_PSEAE
UniRef100 UniRef100_Q9HV66
UniRef50 UniRef50_Q9NUB1
UniRef90 UniRef90_Q9HV66

Product

Feature Type CDS
Coding Frame 1
Product Name
acetyl-coenzyme A synthetase
Product Name Confidence: Class 2
Synonyms acetate-CoA ligase
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -9.26
Kyte-Doolittle Hydrophobicity Value -0.281
Molecular Weight (kDa) 71.6
Isoelectric Point (pI) 6.23

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 586 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000854 (1000 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA4733
Search term: acsB

Human Homologs

Ensembl 110, assembly GRCh38.p14
acyl-CoA synthetase short chain family member 2 [Source:HGNC Symbol;Acc:HGNC:15814]
E-value: 1.7e-217
Percent Identity: 54.8

References

Cloning, characterization, and functional expression of acs, the gene which encodes acetyl coenzyme A synthetase in Escherichia coli.
Kumari S, Tishel R, Eisenbach M, Wolfe AJ
J Bacteriol 1995 May;177(10):2878-86
PubMed ID: 7751300