Pseudomonas aeruginosa PAO1, PA4742 (truB)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA4742
Name
truB
Replicon chromosome
Genomic location 5326018 - 5326932 (- strand)
Transposon Mutants 4 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_253430.1
GI 15599936
Affymetrix PA4742_truB_at
Entrez 881676
GenBank AAG08128.1
INSDC AAB48163.1
NCBI Locus Tag PA4742
protein_id(GenBank) gb|AAG08128.1|AE004888_3|gnl|PseudoCAP|PA4742
TIGR NTL03PA04743
UniParc UPI00001376A5
UniProtKB Acc P72154
UniProtKB ID TRUB_PSEAE
UniRef100 UniRef100_P72154
UniRef50 UniRef50_P72154
UniRef90 UniRef90_P72154

Product

Feature Type CDS
Coding Frame 1
Product Name
tRNA pseudouridine 55 synthase
Product Name Confidence: Class 2
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -0.43
Kyte-Doolittle Hydrophobicity Value -0.207
Molecular Weight (kDa) 33.4
Isoelectric Point (pI) 6.88

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 658 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG004387 (536 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA4742
Search term: truB

Human Homologs

Ensembl 110, assembly GRCh38.p14
TruB pseudouridine synthase family member 1 [Source:HGNC Symbol;Acc:HGNC:16060]
E-value: 8.9e-36
Percent Identity: 39.8

References

Purification, cloning, and properties of the tRNA psi 55 synthase from Escherichia coli.
Nurse K, Wrzesinski J, Bakin A, Lane BG, Ofengand J
RNA 1995 Mar;1(1):102-12
PubMed ID: 7489483