Pseudomonas aeruginosa PAO1, PA4748 (tpiA)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA4748
Name
tpiA
Synonym: tpi
Replicon chromosome
Genomic location 5332746 - 5333501 (- strand)
Transposon Mutants in orthologs 3 transposon mutants in orthologs

Cross-References

RefSeq NP_253436.1
GI 15599942
Affymetrix PA4748_tpiA_at
DNASU PaCD00008060
Entrez 881712
GenBank AAG08134.1
INSDC AAG08134.1
NCBI Locus Tag PA4748
protein_id(GenBank) gb|AAG08134.1|AE004888_9|gnl|PseudoCAP|PA4748
TIGR NTL03PA04749
UniParc UPI000013721C
UniProtKB Acc Q9HV51
UniProtKB ID TPIS_PSEAE
UniRef100 UniRef100_B7V1G0
UniRef50 UniRef50_P50921
UniRef90 UniRef90_A6VCK5

Product

Feature Type CDS
Coding Frame 1
Product Name
triosephosphate isomerase
Product Name Confidence: Class 2
Synonyms TIM
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -4.19
Kyte-Doolittle Hydrophobicity Value 0.303
Molecular Weight (kDa) 25.6
Isoelectric Point (pI) 5.33

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 611 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG004393 (536 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA4748
Search term: tpiA

Human Homologs

Ensembl 110, assembly GRCh38.p14
triosephosphate isomerase 1 [Source:HGNC Symbol;Acc:HGNC:12009]
E-value: 1.0e-45
Percent Identity: 45.8
Ensembl 110, assembly GRCh38.p14
triosephosphate isomerase 1 [Source:HGNC Symbol;Acc:HGNC:12009]
E-value: 1.0e-45
Percent Identity: 45.8
Ensembl 110, assembly GRCh38.p14
triosephosphate isomerase 1 [Source:HGNC Symbol;Acc:HGNC:12009]
E-value: 1.0e-45
Percent Identity: 45.8

References

Replacing the (beta alpha)-unit 8 of E.coli TIM with its chicken homologue leads to a stable and active hybrid enzyme.
Mainfroid V, Goraj K, Rentier-Delrue F, Houbrechts A, Loiseau A, Gohimont AC, Noble ME, Borchert TV, Wierenga RK, Martial JA
Protein Eng 1993 Nov;6(8):893-900
PubMed ID: 8309937
Species-specific inhibition of homologous enzymes by modification of nonconserved amino acids residues. The cysteine residues of triosephosphate isomerase.
Garza-Ramos G, Pérez-Montfort R, Rojo-Domínguez A, de Gómez-Puyou MT, Gómez-Puyou A
Eur J Biochem 1996 Oct 1;241(1):114-20
PubMed ID: 8898895