Pseudomonas aeruginosa PAO1, PA4758 (carA)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA4758
Name
carA
Replicon chromosome
Genomic location 5343767 - 5344903 (- strand)
Transposon Mutants 1 transposon mutants in PAO1
Transposon Mutants in orthologs 2 transposon mutants in orthologs

Cross-References

RefSeq NP_253446.1
GI 15599952
Affymetrix PA4758_carA_at
Entrez 881751
GenBank AAG08144.1
INSDC AAG08144.1
NCBI Locus Tag PA4758
protein_id(GenBank) gb|AAG08144.1|AE004889_7|gnl|PseudoCAP|PA4758
TIGR NTL03PA04759
UniParc UPI0000126F53
UniProtKB Acc P38098
UniProtKB ID CARA_PSEAE
UniRef100 UniRef100_P38098
UniRef50 UniRef50_P38098
UniRef90 UniRef90_P38098

Product

Feature Type CDS
Coding Frame 1
Product Name
carbamoyl-phosphate synthase small chain
Product Name Confidence: Class 1
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -11.23
Kyte-Doolittle Hydrophobicity Value -0.148
Molecular Weight (kDa) 40.8
Isoelectric Point (pI) 5.23

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 623 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG004402 (536 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA4758
Search term: carA

Human Homologs

Ensembl 110, assembly GRCh38.p14
carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase [Source:HGNC Symbol;Acc:HGNC:1424]
E-value: 4.0e-62
Percent Identity: 38.9
Ensembl 110, assembly GRCh38.p14
carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase [Source:HGNC Symbol;Acc:HGNC:1424]
E-value: 4.0e-62
Percent Identity: 38.9

References

Structure and regulation of the carAB operon in Pseudomonas aeruginosa and Pseudomonas stutzeri: no untranslated region exists.
Kwon DH, Lu CD, Walthall DA, Brown TM, Houghton JE, Abdelal AT
J Bacteriol 1994 May;176(9):2532-42
PubMed ID: 8169201