Pseudomonas aeruginosa PAO1, PA4761 (dnaK)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA4761
Name
dnaK
Replicon chromosome
Genomic location 5347198 - 5349111 (- strand)
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_253449.1
GI 15599955
Affymetrix PA4761_dnaK_at
Entrez 881765
GenBank AAG08147.1
INSDC AAG08147.1
NCBI Locus Tag PA4761
protein_id(GenBank) gb|AAG08147.1|AE004889_10|gnl|PseudoCAP|PA4761
TIGR NTL03PA04762
UniParc UPI00001295F4
UniProtKB Acc Q9HV43
UniProtKB ID DNAK_PSEAE
UniRef100 UniRef100_Q02FR1
UniRef50 UniRef50_P38646
UniRef90 UniRef90_Q02FR1

Product

Feature Type CDS
Coding Frame 1
Product Name
DnaK protein
Product Name Confidence: Class 2
Synonyms heat shock protein DnaK
heat shock protein 70, HSP70
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Detected by MALDI-TOF/TOF (PMID:19333994).
Detected using LC-MS/MS (68.40 kDa, PMID:21751344)
Verified using two-dimensional gel electrophoresis, MALDI-TOF MS and peptide mass mapping(pI:4.8, molecular weight:68.4, PMID:21674802)
Charge (pH 7) -29.31
Kyte-Doolittle Hydrophobicity Value -0.326
Molecular Weight (kDa) 68.4
Isoelectric Point (pI) 4.53

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Outer Membrane Vesicle Class 1
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 638 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG004405 (536 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA4761
Search term: dnaK
Search term: DnaK protein

Human Homologs

Ensembl 110, assembly GRCh38.p14
heat shock protein family A (Hsp70) member 9 [Source:HGNC Symbol;Acc:HGNC:5244]
E-value: 5.0e-206
Percent Identity: 62.1

References

Purification and properties of the Escherichia coli dnaK replication protein.
Zylicz M, Georgopoulos C
J Biol Chem 1984 Jul 25;259(14):8820-5
PubMed ID: 6086613
DNA sequence analysis of the dnaK gene of Escherichia coli B and of two dnaK genes carrying the temperature-sensitive mutations dnaK7(Ts) and dnaK756(Ts).
Miyazaki T, Tanaka S, Fujita H, Itikawa H
J Bacteriol 1992 Jun;174(11):3715-22
PubMed ID: 1592823
Inhibition of DnaK autophosphorylation by heat shock proteins and polypeptide substrates.
Panagiotidis CA, Burkholder WF, Gaitanaris GA, Gragerov A, Gottesman ME, Silverstein SJ
J Biol Chem 1994 Jun 17;269(24):16643-7
PubMed ID: 8206983
Structural analysis of substrate binding by the molecular chaperone DnaK.
Zhu X, Zhao X, Burkholder WF, Gragerov A, Ogata CM, Gottesman ME, Hendrickson WA
Science 1996 Jun 14;272(5268):1606-14
PubMed ID: 8658133
NMR solution structure of the 21 kDa chaperone protein DnaK substrate binding domain: a preview of chaperone-protein interaction.
Wang H, Kurochkin AV, Pang Y, Hu W, Flynn GC, Zuiderweg ER
Biochemistry 1998 Jun 2;37(22):7929-40
PubMed ID: 9609686
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994
Proteomics of the oxidative stress response induced by hydrogen peroxide and paraquat reveals a novel AhpC-like protein in Pseudomonas aeruginosa.
Hare NJ, Scott NE, Shin EH, Connolly AM, Larsen MR, Palmisano G, Cordwell SJ
Proteomics 2011 Aug;11(15):3056-69
PubMed ID: 21674802
Proteomic analysis of outer membrane vesicles derived from Pseudomonas aeruginosa.
Choi DS, Kim DK, Choi SJ, Lee J, Choi JP, Rho S, Park SH, Kim YK, Hwang D, Gho YS
Proteomics 2011 Aug;11(16):3424-9
PubMed ID: 21751344
The Pseudomonas aeruginosa dnaK gene is involved in bacterial translocation across the intestinal epithelial cell barrier.
Okuda J, Yamane S, Nagata S, Kunikata C, Suezawa C, Yasuda M
Microbiology (Reading) 2017 Aug;163(8):1208-1216
PubMed ID: 28758636