Pseudomonas aeruginosa PAO1, PA4771 (lldD)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA4771
Name
lldD
Synonym: lctD
Replicon chromosome
Genomic location 5357230 - 5358375 (+ strand)
Transposon Mutants 3 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs
Comment

Cross-References

RefSeq NP_253459.1
GI 15599965
Affymetrix PA4771_lldD_at
DNASU PaCD00008069
Entrez 881809
GenBank AAG08157.1
INSDC AAG08157.1
NCBI Locus Tag PA4771
protein_id(GenBank) gb|AAG08157.1|AE004890_10|gnl|PseudoCAP|PA4771
TIGR NTL03PA04772
UniParc UPI00000C5DF8
UniProtKB Acc Q9HV37
UniProtKB ID LLDD_PSEAE
UniRef100 UniRef100_B7V1I3
UniRef50 UniRef50_C3K053
UniRef90 UniRef90_B7V1I3

Product

Feature Type CDS
Coding Frame 1
Product Name
L-lactate dehydrogenase
Product Name Confidence: Class 1
Synonyms
Evidence for Translation
Detected using LC-MS/MS (41.12 kDa, PMID:21751344)
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -1.82
Kyte-Doolittle Hydrophobicity Value 0.110
Molecular Weight (kDa) 41.1
Isoelectric Point (pI) 6.38

Subcellular localization

Individual Mappings
Localization Confidence PMID
Outer Membrane Vesicle Class 1
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 303 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG004414 (448 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA4771
Search term: lldD

Human Homologs

Ensembl 110, assembly GRCh38.p14
hydroxyacid oxidase 1 [Source:HGNC Symbol;Acc:HGNC:4809]
E-value: 2.6e-56
Percent Identity: 35.5

References

Inducible membrane-bound L-lactate dehydrogenase from Escherichia coli. Purification and properties.
Futai M, Kimura H
J Biol Chem 1977 Aug 25;252(16):5820-7
PubMed ID: 18473
Effects of phospholipids on L-lactate dehydrogenase from membranes of Escherichia coli. Activation and stabilization of the enzyme with phospholipids.
Kimura H, Futai M
J Biol Chem 1978 Feb 25;253(4):1095-110
PubMed ID: 342518
Three overlapping lct genes involved in L-lactate utilization by Escherichia coli.
Dong JM, Taylor JS, Latour DJ, Iuchi S, Lin EC
J Bacteriol 1993 Oct;175(20):6671-8
PubMed ID: 8407843
Proteomic analysis of outer membrane vesicles derived from Pseudomonas aeruginosa.
Choi DS, Kim DK, Choi SJ, Lee J, Choi JP, Rho S, Park SH, Kim YK, Hwang D, Gho YS
Proteomics 2011 Aug;11(16):3424-9
PubMed ID: 21751344
Two NAD-independent l-lactate dehydrogenases drive l-lactate utilization in Pseudomonas aeruginosa PAO1.
Wang Y, Xiao D, Liu Q, Zhang Y, Hu C, Sun J, Yang C, Xu P, Ma C, Gao C
Environ Microbiol Rep 2018 Oct;10(5):569-575
PubMed ID: 30066495