Pseudomonas aeruginosa PAO1, PA4773 (speD2)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA4773
Name
speD2
Replicon chromosome
Genomic location 5361586 - 5362068 (+ strand)
Transposon Mutants 1 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs
CommentSpeD converts S-adenosylmethionine (SAM) to decarboxylated SAM (dSAM), which is used as a substrate with putrescine to synthesize spermidine by SpeE.
CommentRequired for spermidine synthesis on the bacterial surface.

Cross-References

RefSeq NP_253461.1
GI 15599967
Affymetrix PA4773_at
DNASU PaCD00008070
Entrez 881823
GenBank AAG08159.1
INSDC AAG08159.1
NCBI Locus Tag PA4773
protein_id(GenBank) gb|AAG08159.1|AE004891_1|gnl|PseudoCAP|PA4773
TIGR NTL03PA04774
UniParc UPI0000135DFB
UniProtKB Acc Q9HV35
UniProtKB ID SPEH_PSEAE
UniRef100 UniRef100_B7V1I5
UniRef50 UniRef50_B7V1I5
UniRef90 UniRef90_B7V1I5

Product

Feature Type CDS
Coding Frame 1
Product Name
SpeD2
Product Name Confidence: Class 1
Synonyms hypothetical protein
Evidence for Translation
Detected using LC-MS/MS (17.32 kDa, PMID:21751344)
Charge (pH 7) -4.87
Kyte-Doolittle Hydrophobicity Value 0.135
Molecular Weight (kDa) 17.3
Isoelectric Point (pI) 5.17

Subcellular localization

Individual Mappings
Localization Confidence PMID
Unknown Class 3
Outer Membrane Vesicle Class 1
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 218 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG004416 (293 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA4773
Search term: speD2
Search term: SpeD2

Human Homologs

References

Proteomic analysis of outer membrane vesicles derived from Pseudomonas aeruginosa.
Choi DS, Kim DK, Choi SJ, Lee J, Choi JP, Rho S, Park SH, Kim YK, Hwang D, Gho YS
Proteomics 2011 Aug;11(16):3424-9
PubMed ID: 21751344
Surface-localized spermidine protects the Pseudomonas aeruginosa outer membrane from antibiotic treatment and oxidative stress.
Johnson L, Mulcahy H, Kanevets U, Shi Y, Lewenza S
J Bacteriol 2012 Feb;194(4):813-26
PubMed ID: 22155771