Pseudomonas aeruginosa PAO1, PA4774 (speE2)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA4774
Name
speE2
Replicon chromosome
Genomic location 5362146 - 5363195 (+ strand)
Transposon Mutants 3 transposon mutants in PAO1
Transposon Mutants in orthologs 2 transposon mutants in orthologs
Comment

Cross-References

RefSeq NP_253462.1
GI 15599968
Affymetrix PA4774_at
Entrez 881824
GenBank AAG08160.1
INSDC AAG08160.1
NCBI Locus Tag PA4774
protein_id(GenBank) gb|AAG08160.1|AE004891_2|gnl|PseudoCAP|PA4774
TIGR NTL03PA04775
UniParc UPI00000C5DFA
UniProtKB Acc Q9HV34
UniProtKB ID SPEE2_PSEAE
UniRef100 UniRef100_Q9HV34
UniRef50 UniRef50_Q9HV34
UniRef90 UniRef90_Q9HV34

Product

Feature Type CDS
Coding Frame 1
Product Name
SpeE2
Product Name Confidence: Class 1
Synonyms hypothetical protein
Evidence for Translation
Charge (pH 7) -24.19
Kyte-Doolittle Hydrophobicity Value -0.218
Molecular Weight (kDa) 39.5
Isoelectric Point (pI) 4.44

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 1
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 351 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG003702 (713 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA4774
Search term: speE2
Search term: SpeE2

Human Homologs

Ensembl 110, assembly GRCh38.p14
spermidine synthase [Source:HGNC Symbol;Acc:HGNC:11296]
E-value: 4.0e-32
Percent Identity: 30.0

References

Surface-localized spermidine protects the Pseudomonas aeruginosa outer membrane from antibiotic treatment and oxidative stress.
Johnson L, Mulcahy H, Kanevets U, Shi Y, Lewenza S
J Bacteriol 2012 Feb;194(4):813-26
PubMed ID: 22155771