Pseudomonas aeruginosa PAO1, PA4835 (cntM)

Gene Feature Overview

Strain	Pseudomonas aeruginosa PAO1 (Stover et al., 2000) GCF_000006765.1|latest
Locus Tag	PA4835
Name	cntM Synonym: zrmC
Replicon	chromosome
Genomic location	5425597 - 5426898 (- strand)
Transposon Mutants	2 transposon mutants in PAO1
Transposon Mutants in orthologs	1 transposon mutants in orthologs
Comment	part of the Zur-regulated metallophore operon known as cntOLMI (PMID:29214991) or zrmABCD(PMID:28898501).

Cross-References

RefSeq	NP_253522.1
GI	15600028
Affymetrix	PA4835_at
Entrez	882352
GenBank	AAG08220.1
INSDC	AAG08220.1
NCBI Locus Tag	PA4835
protein_id(GenBank)	gb|AAG08220.1|AE004896_10|gnl|PseudoCAP|PA4835
TIGR	NTL03PA04836
UniParc	UPI00000C5E30
UniProtKB Acc	Q9HUX5
UniProtKB ID	Q9HUX5_PSEAE
UniRef100	UniRef100_Q9HUX5
UniRef50	UniRef50_Q9HUX5
UniRef90	UniRef90_Q9HUX5

Product

Feature Type	CDS
Coding Frame	1
Product Name	CntM Product Name Confidence: Class 3
Synonyms	hypothetical protein
Evidence for Translation	Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7)	0.90
Kyte-Doolittle Hydrophobicity Value	-0.053
Molecular Weight (kDa)	47.7
Isoelectric Point (pI)	7.21

Subcellular localization

Individual Mappings	Localization Confidence PMID Unknown Class 3 20472543
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession	Header	Accession Date	Compound	Source	Resolution	Method	Percent Identity
6PBN	BIOSYNTHETIC PROTEIN	06/14/19	Pseudopaline Dehydrogenase with (R)-Pseudopaline Soaked 1 hour	Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)	1.65	X-RAY DIFFRACTION	100.0
6PBT	BIOSYNTHETIC PROTEIN	06/14/19	Pseudopaline Dehydrogenase with (R)-Pseudopaline Soaked 2 hours	Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)	2.18	X-RAY DIFFRACTION	100.0
6PBP	BIOSYNTHETIC PROTEIN	06/14/19	Pseudopaline Dehydrogenase with (S)-Pseudopaline Soaked 1 hour	Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)	1.64	X-RAY DIFFRACTION	100.0
6C4N	OXIDOREDUCTASE	01/12/18	Pseudopaline dehydrogenase (PaODH) - NADP+ bound	Pseudomonas aeruginosa PAO1	1.95	X-RAY DIFFRACTION	100.0
6PBM	oxidoreductase, biosynthetic protein	06/14/19	Pseudopaline Dehydrogenase with NADP+ bound	Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)	1.57	X-RAY DIFFRACTION	100.0

Pathogen Association Analysis

Results

Common Found in both pathogen and nonpathogenic strains Hits to this gene were found in 11 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database	View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group	POG004466 (299 members)
Putative Inparalogs	None Found

Interactions

STRING database

Search for predicted protein-protein interactions using: Search term: PA4835 Search term: cntM Search term: CntM

Human Homologs

References

Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen. Lewenza S, Gardy JL, Brinkman FS, Hancock RE Genome Res 2005 Feb;15(2):321-9 PubMed ID: 15687295

Growth of Pseudomonas aeruginosa in zinc poor environments is promoted by a nicotianamine-related metallophore. Mastropasqua MC, D'Orazio M, Cerasi M, Pacello F, Gismondi A, Canini A, Canuti L, Consalvo A, Ciavardelli D, Chirullo B, Pasquali P, Battistoni A Mol Microbiol 2017 Nov;106(4):543-561 PubMed ID: 28898501

Pseudomonas aeruginosa zinc uptake in chelating environment is primarily mediated by the metallophore pseudopaline. Lhospice S, Gomez NO, Ouerdane L, Brutesco C, Ghssein G, Hajjar C, Liratni A, Wang S, Richaud P, Bleves S, Ball G, Borezée-Durant E, Lobinski R, Pignol D, Arnoux P, Voulhoux R Sci Rep 2017 Dec 7;7(1):17132 PubMed ID: 29214991