Pseudomonas aeruginosa PAO1, PA4847 (accB)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA4847
Name
accB
Synonym: fabE
Replicon chromosome
Genomic location 5442304 - 5442774 (+ strand)

Cross-References

RefSeq NP_253534.1
GI 15600040
Affymetrix PA4847_accB_at
Entrez 879557
GenBank AAG08232.1
INSDC AAG08232.1
NCBI Locus Tag PA4847
protein_id(GenBank) gb|AAG08232.1|AE004898_3|gnl|PseudoCAP|PA4847
TIGR NTL03PA04848
UniParc UPI00001267E6
UniProtKB Acc P37799
UniProtKB ID BCCP_PSEAE
UniRef100 UniRef100_P37799
UniRef50 UniRef50_P37799
UniRef90 UniRef90_P37799

Product

Feature Type CDS
Coding Frame 1
Product Name
biotin carboxyl carrier protein (BCCP)
Product Name Confidence: Class 1
Synonyms acetyl-CoA carboxylase
Evidence for Translation
Verified using two-dimensional gel electrophoresis, MALDI-TOF MS and peptide mass mapping(pI:5.0, molecular weight:16.5, PMID:21674802)
Detected by two-dimensional gel electrophoresis and mass spectral analysis(PMID:21863142).
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -5.55
Kyte-Doolittle Hydrophobicity Value 0.068
Molecular Weight (kDa) 16.5
Isoelectric Point (pI) 4.67

Subcellular localization

Individual Mappings
Localization Confidence PMID
Unknown (This protein may have multiple localization sites) Class 3
Unknown (This protein may have multiple localization sites) Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 521 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG004476 (535 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA4847
Search term: accB

Human Homologs

References

Organization and nucleotide sequences of the genes encoding the biotin carboxyl carrier protein and biotin carboxylase protein of Pseudomonas aeruginosa acetyl coenzyme A carboxylase.
Best EA, Knauf VC
J Bacteriol 1993 Nov;175(21):6881-9
PubMed ID: 7693652
Comparisons of Two Proteomic Analyses of Non-Mucoid and Mucoid Pseudomonas aeruginosa Clinical Isolates from a Cystic Fibrosis Patient.
Rao J, Damron FH, Basler M, Digiandomenico A, Sherman NE, Fox JW, Mekalanos JJ, Goldberg JB
Front Microbiol 2011;2:162
PubMed ID: 21863142
Proteomics of the oxidative stress response induced by hydrogen peroxide and paraquat reveals a novel AhpC-like protein in Pseudomonas aeruginosa.
Hare NJ, Scott NE, Shin EH, Connolly AM, Larsen MR, Palmisano G, Cordwell SJ
Proteomics 2011 Aug;11(15):3056-69
PubMed ID: 21674802