Pseudomonas aeruginosa PAO1, PA4866

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA4866
Name
Replicon chromosome
Genomic location 5463918 - 5464436 (+ strand)
Transposon Mutants 2 transposon mutants in PAO1

Cross-References

RefSeq NP_253553.1
GI 15600059
Affymetrix PA4866_at
Entrez 882184
GenBank AAG08251.1
INSDC AAG08251.1
NCBI Locus Tag PA4866
protein_id(GenBank) gb|AAG08251.1|AE004900_5|gnl|PseudoCAP|PA4866
TIGR NTL03PA04867
UniParc UPI00000C5E48
UniProtKB Acc Q9HUU7
UniProtKB ID Q9HUU7_PSEAE
UniRef100 UniRef100_Q9HUU7
UniRef50 UniRef50_D5RSX7
UniRef90 UniRef90_Q9HUU7

Product

Feature Type CDS
Coding Frame 1
Product Name
putative phosphinothricin acetyltransferase
Product Name Confidence: Class 3
Synonyms pita
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -1.29
Kyte-Doolittle Hydrophobicity Value -0.154
Molecular Weight (kDa) 18.7
Isoelectric Point (pI) 6.26

Subcellular localization

Individual Mappings
Localization Confidence PMID
Unknown Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
2BL1 TRANSFERASE 02/24/05 Crystal structure of a putative phosphinothricin Acetyltransferase (PA4866) from Pseudomonas aeruginosa PAC1 PSEUDOMONAS AERUGINOSA 2 X-RAY DIFFRACTION 99.4
1YVO TRANSFERASE 02/16/05 hypothetical acetyltransferase from P.aeruginosa PA01 Pseudomonas aeruginosa 1.9 X-RAY DIFFRACTION 100.0
2J8M TRANSFERASE 10/26/06 Structure of P. aeruginosa acetyltransferase PA4866 PSEUDOMONAS AERUGINOSA 1.44 X-RAY DIFFRACTION 99.4
2J8N TRANSFERASE 10/26/06 Structure of P. aeruginosa acetyltransferase PA4866 solved at room temperature PSEUDOMONAS AERUGINOSA 2.35 X-RAY DIFFRACTION 99.4
2J8R TRANSFERASE 10/27/06 Structure of P. aeruginosa acetyltransferase PA4866 solved in complex with L-Methionine sulfoximine PSEUDOMONAS AERUGINOSA 1.55 X-RAY DIFFRACTION 99.4

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 324 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG004494 (530 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA4866

Human Homologs

References

Crystal structure of a putative phosphinothricin acetyltransferase (PA4866) from Pseudomonas aeruginosa PAC1.
Davies AM, Tata R, Agha R, Sutton BJ, Brown PR
Proteins 2005 Nov 15;61(3):677-9
PubMed ID: 16161106