Pseudomonas aeruginosa PAO1, PA4930 (alr)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA4930
Name
alr
Replicon chromosome
Genomic location 5533017 - 5534093 (- strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 6 transposon mutants in orthologs
Comment

Cross-References

RefSeq NP_253617.1
GI 15600123
Affymetrix PA4930_alr_at
DNASU PaCD00008135
Entrez 878646
GenBank AAG08315.1
INSDC AAG08315.1
NCBI Locus Tag PA4930
protein_id(GenBank) gb|AAG08315.1|AE004906_3|gnl|PseudoCAP|PA4930
TIGR NTL03PA04931
UniParc UPI00001258C3
UniProtKB Acc Q9HUN4
UniProtKB ID ALR1_PSEAE
UniRef100 UniRef100_Q9HUN4
UniRef50 UniRef50_Q9HUN4
UniRef90 UniRef90_Q9HUN4

Product

Feature Type CDS
Coding Frame 1
Product Name
biosynthetic alanine racemase
Product Name Confidence: Class 1
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -5.53
Kyte-Doolittle Hydrophobicity Value 0.128
Molecular Weight (kDa) 38.3
Isoelectric Point (pI) 6.13

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
6A2F ISOMERASE 06/11/18 Crystal structure of biosynthetic alanine racemase from Pseudomonas aeruginosa Pseudomonas aeruginosa PAO1 2.5 X-RAY DIFFRACTION 100.0

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 558 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG004545 (850 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA4930
Search term: alr

Human Homologs

References

Biosynthetic alr alanine racemase from Salmonella typhimurium: DNA and protein sequence determination.
Galakatos NG, Daub E, Botstein D, Walsh CT
Biochemistry 1986 Jun 3;25(11):3255-60
PubMed ID: 3524676
Correlation between catalytic activity and monomer-dimer equilibrium of bacterial alanine racemases.
Ju J, Xu S, Furukawa Y, Zhang Y, Misono H, Minamino T, Namba K, Zhao B, Ohnishi K
J Biochem 2011 Jan;149(1):83-9
PubMed ID: 20971724
Enzymatic characterization and crystal structure of biosynthetic alanine racemase from Pseudomonas aeruginosa PAO1.
Dong H, Han Q, Guo Y, Ju J, Wang S, Yuan C, Long W, He X, Xu S, Li S
Biochem Biophys Res Commun 2018 Sep 18;503(4):2319-2325
PubMed ID: 29964014