Pseudomonas aeruginosa PAO1, PA4934 (rpsR)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA4934
Name
rpsR
Replicon chromosome
Genomic location 5537059 - 5537289 (- strand)
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_253621.1
GI 15600127
Affymetrix PA4934_rpsR_at
DNASU PaCD00008137
Entrez 878035
GenBank AAG08319.1
INSDC AAG08319.1
NCBI Locus Tag PA4934
protein_id(GenBank) gb|AAG08319.1|AE004906_7|gnl|PseudoCAP|PA4934
TIGR NTL03PA04935
UniParc UPI0000134DAD
UniProtKB Acc Q9HUN0
UniProtKB ID RS18_PSEAE
UniRef100 UniRef100_A6VD47
UniRef50 UniRef50_Q6N5A3
UniRef90 UniRef90_C1DLR3

Product

Feature Type CDS
Coding Frame 1
Product Name
30S ribosomal protein S18
Product Name Confidence: Class 2
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) 12.19
Kyte-Doolittle Hydrophobicity Value -0.607
Molecular Weight (kDa) 8.9
Isoelectric Point (pI) 11.06

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
7UNR RIBOSOME 04/11/22 Pseudomonas aeruginosa 70S ribosome initiation complex bound to compact IF2-GDP (composite structure I-A) Escherichia coli; Pseudomonas aeruginosa PAO1; SYNTHETIC CONSTRUCT 2.9 ELECTRON MICROSCOPY 100.0
6SPE RIBOSOME 09/01/19 Pseudomonas aeruginosa 30s ribosome from a clinical isolate Pseudomonas aeruginosa 3.6 ELECTRON MICROSCOPY 98.6
6SPG RIBOSOME 09/01/19 Pseudomonas aeruginosa 70s ribosome from a clinical isolate Pseudomonas aeruginosa 3.34 ELECTRON MICROSCOPY 98.6
7UNU RIBOSOME 04/11/22 Pseudomonas aeruginosa 70S ribosome initiation complex bound to compact IF2-GDP (composite structure I-B) Escherichia coli; Pseudomonas aeruginosa PAO1; SYNTHETIC CONSTRUCT 2.9 ELECTRON MICROSCOPY 100.0
7UNV RIBOSOME 04/11/22 Pseudomonas aeruginosa 70S ribosome initiation complex bound to IF2-GDPCP (structure II-A) Escherichia coli; Pseudomonas aeruginosa PAO1; SYNTHETIC CONSTRUCT 2.7 ELECTRON MICROSCOPY 100.0
7UNW RIBOSOME 04/11/22 Pseudomonas aeruginosa 70S ribosome initiation complex bound to IF2-GDPCP (structure II-B) Escherichia coli; Pseudomonas aeruginosa PAO1; SYNTHETIC CONSTRUCT 2.6 ELECTRON MICROSCOPY 100.0

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 588 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG004549 (536 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA4934
Search term: rpsR

Human Homologs

References

Primary structure of protein S18 from the small Escherichia coli ribosomal subunit.
Yaguchi M
FEBS Lett 1975 Nov 15;59(2):217-20
PubMed ID: 776663
The nucleotide sequence of an Escherichia coli chromosomal region containing the genes for ribosomal proteins S6, S18, L9 and an open reading frame.
Schnier J, Kitakawa M, Isono K
Mol Gen Genet 1986 Jul;204(1):126-32
PubMed ID: 3528756
Cloning and nucleotide sequencing of the genes rimI and rimJ which encode enzymes acetylating ribosomal proteins S18 and S5 of Escherichia coli K12.
Yoshikawa A, Isono S, Sheback A, Isono K
Mol Gen Genet 1987 Oct;209(3):481-8
PubMed ID: 2828880
Interaction of ribosomal proteins S5, S6, S11, S12, S18 and S21 with 16 S rRNA.
Stern S, Powers T, Changchien LM, Noller HF
J Mol Biol 1988 Jun 20;201(4):683-95
PubMed ID: 2459389