Pseudomonas aeruginosa PAO1, PA4935 (rpsF)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA4935
Name
rpsF
Replicon chromosome
Genomic location 5537319 - 5537738 (- strand)

Cross-References

RefSeq NP_253622.1
GI 15600128
Affymetrix PA4935_rpsF_at
DNASU PaCD00008138
Entrez 877646
GenBank AAG08320.1
INSDC AAG08320.1
NCBI Locus Tag PA4935
protein_id(GenBank) gb|AAG08320.1|AE004906_8|gnl|PseudoCAP|PA4935
TIGR NTL03PA04936
UniParc UPI00001350C1
UniProtKB Acc Q9HUM9
UniProtKB ID RS6_PSEAE
UniRef100 UniRef100_A6VD48
UniRef50 UniRef50_P02358
UniRef90 UniRef90_A6VD48

Product

Feature Type CDS
Coding Frame 1
Product Name
30S ribosomal protein S6
Product Name Confidence: Class 2
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Detected by MALDI-TOF/TOF (PMID:19333994).
Charge (pH 7) -10.82
Kyte-Doolittle Hydrophobicity Value -1.088
Molecular Weight (kDa) 16.2
Isoelectric Point (pI) 4.58

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
6SPC RIBOSOME 09/01/19 Pseudomonas aeruginosa 30s ribosome from an aminoglycoside resistant clinical isolate Pseudomonas aeruginosa 2.95 ELECTRON MICROSCOPY 99.0
7UNV RIBOSOME 04/11/22 Pseudomonas aeruginosa 70S ribosome initiation complex bound to IF2-GDPCP (structure II-A) Escherichia coli; Pseudomonas aeruginosa PAO1; SYNTHETIC CONSTRUCT 2.7 ELECTRON MICROSCOPY 100.0
6SPE RIBOSOME 09/01/19 Pseudomonas aeruginosa 30s ribosome from a clinical isolate Pseudomonas aeruginosa 3.6 ELECTRON MICROSCOPY 100.0
7UNW RIBOSOME 04/11/22 Pseudomonas aeruginosa 70S ribosome initiation complex bound to IF2-GDPCP (structure II-B) Escherichia coli; Pseudomonas aeruginosa PAO1; SYNTHETIC CONSTRUCT 2.6 ELECTRON MICROSCOPY 100.0
6SPF RIBOSOME 09/01/19 Pseudomonas aeruginosa 70s ribosome from an aminoglycoside resistant clinical isolate Pseudomonas aeruginosa 2.89 ELECTRON MICROSCOPY 99.0
7UNR RIBOSOME 04/11/22 Pseudomonas aeruginosa 70S ribosome initiation complex bound to compact IF2-GDP (composite structure I-A) Escherichia coli; Pseudomonas aeruginosa PAO1; SYNTHETIC CONSTRUCT 2.9 ELECTRON MICROSCOPY 100.0
7UNU RIBOSOME 04/11/22 Pseudomonas aeruginosa 70S ribosome initiation complex bound to compact IF2-GDP (composite structure I-B) Escherichia coli; Pseudomonas aeruginosa PAO1; SYNTHETIC CONSTRUCT 2.9 ELECTRON MICROSCOPY 100.0
6SPG RIBOSOME 09/01/19 Pseudomonas aeruginosa 70s ribosome from a clinical isolate Pseudomonas aeruginosa 3.34 ELECTRON MICROSCOPY 100.0

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 555 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG004550 (536 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA4935
Search term: rpsF

Human Homologs

References

A new ribosomal protein locus in Escherichia coli: the gene for protein S6 maps at 97 min.
Isono K, Kitakawa M
Mol Gen Genet 1977 Jun 8;153(2):115-20
PubMed ID: 329109
Characterization of the gene rimK responsible for the addition of glutamic acid residues to the C-terminus of ribosomal protein S6 in Escherichia coli K12.
Kang WK, Icho T, Isono S, Kitakawa M, Isono K
Mol Gen Genet 1989 Jun;217(2-3):281-8
PubMed ID: 2570347
Phosphorylation of elongation factor G and ribosomal protein S6 in bacteriophage T7-infected Escherichia coli.
Robertson ES, Aggison LA, Nicholson AW
Mol Microbiol 1994 Mar;11(6):1045-57
PubMed ID: 8022276
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994