Pseudomonas aeruginosa PAO1, PA4953 (motB)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA4953
Name
motB
Synonym: rpmB
Replicon chromosome
Genomic location 5557958 - 5559001 (- strand)
Transposon Mutants 3 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs
CommentrpmA and rpmB gene products are required for the efficient ingestion of P. aeruginosa by macrophages.

Cross-References

RefSeq NP_253640.1
GI 15600146
Affymetrix PA4953_motB_at
Entrez 878217
GenBank AAG08338.1
INSDC AAG08338.1
NCBI Locus Tag PA4953
protein_id(GenBank) gb|AAG08338.1|AE004908_4|gnl|PseudoCAP|PA4953
TIGR NTL03PA04954
UniParc UPI00000C5E90
UniProtKB Acc Q9HUL2
UniProtKB ID Q9HUL2_PSEAE
UniRef100 UniRef100_W0Z111
UniRef50 UniRef50_Q4KJ83
UniRef90 UniRef90_A6VD66

Product

Feature Type CDS
Coding Frame 1
Product Name
chemotaxis protein MotB
Product Name Confidence: Class 2
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -9.78
Kyte-Doolittle Hydrophobicity Value -0.448
Molecular Weight (kDa) 37.7
Isoelectric Point (pI) 4.83

Subcellular localization

Individual Mappings
Localization Confidence PMID
Unknown (This protein may have multiple localization sites) Class 3
Cytoplasmic Membrane Class 2
Unknown (This protein may have multiple localization sites) Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Virulence Evidence

Source 1
Database VFDB
Category Flagella
Evidence
Host Organism
Notes
Reference 10768936

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 274 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG004568 (536 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA4953
Search term: motB

Human Homologs

References

RpmA is required for nonopsonic phagocytosis of Pseudomonas aeruginosa.
Simpson DA, Speert DP
Infect Immun 2000 May;68(5):2493-502
PubMed ID: 10768936
Evidence for two flagellar stators and their role in the motility of Pseudomonas aeruginosa.
Toutain CM, Zegans ME, O'Toole GA
J Bacteriol 2005 Jan;187(2):771-7
PubMed ID: 15629949
Mutant MotB proteins in Escherichia coli.
Blair DF, Kim DY, Berg HC
J Bacteriol 1991 Jul;173(13):4049-55
PubMed ID: 2061285