Pseudomonas aeruginosa PAO1, PA4958 (fimW)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA4958
Name
fimW
Replicon chromosome
Genomic location 5563605 - 5565428 (+ strand)
Transposon Mutants 3 transposon mutants in PAO1
Transposon Mutants in orthologs 3 transposon mutants in orthologs
CommentA pilZ domain starting at amino acid 323 and stopping at amino acid 492 was manually validated
CommentFimW is a cyclic-di-GMP receptor that regulates Type IV pili elongation.
CommentSite-directed mutagenesis demonstrated that the ligand (cyclic-di-GMP) binding motif is R324, R328, N435, S437, G440.

Cross-References

RefSeq NP_253645.1
GI 15600151
Affymetrix PA4958_at
Entrez 878415
GenBank AAG08343.1
INSDC AAG08343.1
NCBI Locus Tag PA4958
protein_id(GenBank) gb|AAG08343.1|AE004908_9|gnl|PseudoCAP|PA4958
TIGR NTL03PA04959
UniParc UPI00000C5E95
UniProtKB Acc Q9HUK7
UniProtKB ID Q9HUK7_PSEAE
UniRef100 UniRef100_Q9HUK7
UniRef50 UniRef50_Q9HUK7
UniRef90 UniRef90_Q9HUK7

Product

Feature Type CDS
Coding Frame 1
Product Name
FimW
Product Name Confidence: Class 1
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) 2.80
Kyte-Doolittle Hydrophobicity Value -0.249
Molecular Weight (kDa) 67.8
Isoelectric Point (pI) 7.42

Subcellular localization

Individual Mappings
Localization Confidence PMID
Unknown Class 3
Cytoplasmic Class 1
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 24 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG004573 (412 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA4958
Search term: fimW
Search term: FimW

Human Homologs

References

A Surface-Induced Asymmetric Program Promotes Tissue Colonization by Pseudomonas aeruginosa.
Laventie BJ, Sangermani M, Estermann F, Manfredi P, Planes R, Hug I, Jaeger T, Meunier E, Broz P, Jenal U
Cell Host Microbe 2019 Jan 9;25(1):140-152.e6
PubMed ID: 30581112