Pseudomonas aeruginosa PAO1, PA4985

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA4985
Name
Replicon chromosome
Genomic location 5599884 - 5601131 (- strand)
Transposon Mutants 2 transposon mutants in PAO1
CommentTranslation confirmed using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer. Coordinates verified using RT-PCR (PMID:24291602).

Cross-References

RefSeq NP_253672.1
GI 15600178
Affymetrix PA4985_at
Entrez 880154
GenBank AAG08370.1
INSDC AAG08370.1
NCBI Locus Tag PA4985
protein_id(GenBank) gb|AAG08370.1|AE004911_5|gnl|PseudoCAP|PA4985
TIGR NTL03PA04986
UniParc UPI00000C5EAE
UniProtKB Acc Q9HUI0
UniProtKB ID Q9HUI0_PSEAE
UniRef100 UniRef100_Q9HUI0
UniRef50 UniRef50_Q9HUI0
UniRef90 UniRef90_Q9HUI0

Product

Feature Type CDS
Coding Frame 1
Product Name
Uncharacterized protein
Product Name Confidence: Class 1
Synonyms hypothetical protein
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -3.13
Kyte-Doolittle Hydrophobicity Value -0.218
Molecular Weight (kDa) 39.8
Isoelectric Point (pI) 5.78

Subcellular localization

Individual Mappings
Localization Confidence PMID
Periplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 111 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG004597 (348 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA4985

Human Homologs

References

LTQ-XL mass spectrometry proteome analysis expands the Pseudomonas aeruginosa AmpR regulon to include cyclic di-GMP phosphodiesterases and phosphoproteins, and identifies novel open reading frames.
Kumari H, Murugapiran SK, Balasubramanian D, Schneper L, Merighi M, Sarracino D, Lory S, Mathee K
J Proteomics 2014 Jan 16;96:328-342
PubMed ID: 24291602