Pseudomonas aeruginosa PAO1, PA4991

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA4991
Name
Replicon chromosome
Genomic location 5606496 - 5607671 (+ strand)
CommentFrom publication: Analysis of the structure of PA4991 identifies this enzyme as a member of the GR2 family of flavoenzymes. Enzymatic assays did not support a function as a G3P dehydrogenase/oxidase or an amino-acid oxidase as hypothesized based on the three-dimensional structure

Cross-References

RefSeq NP_253678.1
GI 15600184
Affymetrix PA4991_at
Entrez 880166
GenBank AAG08376.1
INSDC AAG08376.1
NCBI Locus Tag PA4991
protein_id(GenBank) gb|AAG08376.1|AE004912_2|gnl|PseudoCAP|PA4991
TIGR NTL03PA04992
UniParc UPI00000C5EB4
UniProtKB Acc Q9HUH4
UniProtKB ID Q9HUH4_PSEAE
UniRef100 UniRef100_Q9HUH4
UniRef50 UniRef50_Q4KJA6
UniRef90 UniRef90_Q9HUH4

Product

Feature Type CDS
Coding Frame 1
Product Name
FAD dependent oxidoreductase
Product Name Confidence: Class 1
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) 2.58
Kyte-Doolittle Hydrophobicity Value -0.130
Molecular Weight (kDa) 42.1
Isoelectric Point (pI) 8.00

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
5EZ7 OXIDOREDUCTASE 11/26/15 Crystal structure of the FAD dependent oxidoreductase PA4991 from Pseudomonas aeruginosa Pseudomonas aeruginosa PAO1 2.4 X-RAY DIFFRACTION 100.0

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 13 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG004601 (538 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA4991

Human Homologs

References

Crystal structure of the flavoenzyme PA4991 from Pseudomonas aeruginosa.
Jacewicz A, Schnell R, Lindqvist Y, Schneider G
Acta Crystallogr F Struct Biol Commun 2016 Feb;72(Pt 2):105-11
PubMed ID: 26841760