Pseudomonas aeruginosa PAO1, PA5025 (metY)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA5025
Name
metY
Replicon chromosome
Genomic location 5655648 - 5656925 (+ strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 4 transposon mutants in orthologs

Cross-References

RefSeq NP_253712.1
GI 15600218
Affymetrix PA5025_metY_at
Entrez 881205
GenBank AAG08410.1
INSDC AAG08410.1
NCBI Locus Tag PA5025
protein_id(GenBank) gb|AAG08410.1|AE004915_5|gnl|PseudoCAP|PA5025
TIGR NTL03PA05026
UniParc UPI00000C5ED0
UniProtKB Acc Q9HUE4
UniProtKB ID Q9HUE4_PSEAE
UniRef100 UniRef100_Q9HUE4
UniRef50 UniRef50_Q5LTI3
UniRef90 UniRef90_Q9HUE4

Product

Feature Type CDS
Coding Frame 1
Product Name
homocysteine synthase
Product Name Confidence: Class 2
Synonyms O-acetyl-L-homoserine sulfhydrylase
O-acetylhomoserine (thiol)-lyase
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -4.31
Kyte-Doolittle Hydrophobicity Value 0.132
Molecular Weight (kDa) 45.2
Isoelectric Point (pI) 6.51

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Cytoplasmic Class 2
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 567 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000391 (846 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA5025
Search term: metY

Human Homologs

Ensembl 110, assembly GRCh38.p14
cystathionine gamma-lyase [Source:HGNC Symbol;Acc:HGNC:2501]
E-value: 4.9e-56
Percent Identity: 36.1

References

Direct sulfhydrylation for methionine biosynthesis in Leptospira meyeri.
Belfaiza J, Martel A, Margarita D, Saint Girons I
J Bacteriol 1998 Jan;180(2):250-5
PubMed ID: 9440513