Pseudomonas aeruginosa PAO1, PA5042 (pilO)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA5042
Name
pilO
Replicon chromosome
Genomic location 5678433 - 5679056 (- strand)
Transposon Mutants 3 transposon mutants in PAO1
Transposon Mutants in orthologs 2 transposon mutants in orthologs

Cross-References

RefSeq NP_253729.1
GI 15600235
Affymetrix PA5042_pilO_at
DNASU PaCD00008170
Entrez 880985
GenBank AAG08427.1
INSDC AAG08427.1
NCBI Locus Tag PA5042
protein_id(GenBank) gb|AAG08427.1|AE004917_6|gnl|PseudoCAP|PA5042
TIGR NTL03PA05043
UniParc UPI00000D4260
UniProtKB Acc G3XD51
UniProtKB ID G3XD51_PSEAE
UniRef100 UniRef100_W0Z1B7
UniRef50 UniRef50_Q4KJJ2
UniRef90 UniRef90_Q02EX5

Product

Feature Type CDS
Coding Frame 1
Product Name
type 4 fimbrial biogenesis protein PilO
Product Name Confidence: Class 1
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -6.31
Kyte-Doolittle Hydrophobicity Value -0.004
Molecular Weight (kDa) 22.8
Isoelectric Point (pI) 4.78

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Membrane Class 3
Cytoplasmic Membrane Class 1
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
5UVR MEMBRANE PROTEIN 02/20/17 The core region of PilO from the type IV pilus system of Pseudomonas aeruginosa Pseudomonas aeruginosa 1.7 X-RAY DIFFRACTION 100.0
2RJZ STRUCTURAL GENOMICS, UNKNOWN FUNCTION 10/16/07 Crystal structure of the type 4 fimbrial biogenesis protein PilO from Pseudomonas aeruginosa Pseudomonas aeruginosa 2.2 X-RAY DIFFRACTION 99.3

Virulence Evidence

Source 1
Database VFDB : VF0082
Category Type IV pili biosynthesis
Evidence
Host Organism
Notes
Reference

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 112 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG004645 (941 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA5042
Search term: pilO

Human Homologs

References

Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res 2005 Feb;15(2):321-9
PubMed ID: 15687295
Characterization of a five-gene cluster required for the biogenesis of type 4 fimbriae in Pseudomonas aeruginosa.
Martin PR, Watson AA, McCaul TF, Mattick JS
Mol Microbiol 1995 May;16(3):497-508
PubMed ID: 7565110
Characterization of genes required for pilus expression in Pseudomonas syringae pathovar phaseolicola.
Roine E, Nunn DN, Paulin L, Romantschuk M
J Bacteriol 1996 Jan;178(2):410-7
PubMed ID: 8550460
PilM/N/O/P proteins form an inner membrane complex that affects the stability of the Pseudomonas aeruginosa type IV pilus secretin.
Ayers M, Sampaleanu LM, Tammam S, Koo J, Harvey H, Howell PL, Burrows LL
J Mol Biol 2009 Nov 20;394(1):128-42
PubMed ID: 19857645