Pseudomonas aeruginosa PAO1, PA5050 (priA)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA5050
Name
priA
Replicon chromosome
Genomic location 5687496 - 5689715 (+ strand)

Cross-References

RefSeq NP_253737.1
GI 15600243
Affymetrix PA5050_priA_at
Entrez 881150
GenBank AAG08435.1
INSDC AAG08435.1
NCBI Locus Tag PA5050
protein_id(GenBank) gb|AAG08435.1|AE004918_5|gnl|PseudoCAP|PA5050
TIGR NTL03PA05051
UniParc UPI00000C5EDE
UniProtKB Acc Q9HUC9
UniProtKB ID Q9HUC9_PSEAE
UniRef100 UniRef100_Q9HUC9
UniRef50 UniRef50_P17888
UniRef90 UniRef90_Q9HUC9

Product

Feature Type CDS
Coding Frame 1
Product Name
primosomal protein N'
Product Name Confidence: Class 2
Synonyms replication factor Y
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) 16.62
Kyte-Doolittle Hydrophobicity Value -0.260
Molecular Weight (kDa) 82.1
Isoelectric Point (pI) 8.83

Subcellular localization

Individual Mappings
Localization Confidence PMID
Unknown (This protein may have multiple localization sites) Class 3
Unknown (This protein may have multiple localization sites) Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 580 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG004652 (536 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA5050
Search term: priA

Human Homologs

References

Molecular cloning and DNA sequence analysis of Escherichia coli priA, the gene encoding the primosomal protein replication factor Y.
Nurse P, DiGate RJ, Zavitz KH, Marians KJ
Proc Natl Acad Sci U S A 1990 Jun;87(12):4615-9
PubMed ID: 2162049
The priA gene encoding the primosomal replicative n' protein of Escherichia coli.
Lee EH, Masai H, Allen GC Jr, Kornberg A
Proc Natl Acad Sci U S A 1990 Jun;87(12):4620-4
PubMed ID: 2162050
Bacterial DNA replication initiation factor priA is related to proteins belonging to the 'DEAD-box' family.
Ouzounis CA, Blencowe BJ
Nucleic Acids Res 1991 Dec 25;19(24):6953
PubMed ID: 1662369