Pseudomonas aeruginosa PAO1, PA5077 (opgH)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA5077
Name
opgH
Synonym: mdoH
Replicon chromosome
Genomic location 5714270 - 5716855 (- strand)
Transposon Mutants 5 transposon mutants in PAO1
Transposon Mutants in orthologs 3 transposon mutants in orthologs

Cross-References

RefSeq NP_253764.1
GI 15600270
Affymetrix PA5077_mdoH_at
Entrez 879628
GenBank AAG08462.1
INSDC AAG08462.1
NCBI Locus Tag PA5077
protein_id(GenBank) gb|AAG08462.1|AE004920_12|gnl|PseudoCAP|PA5077
TIGR NTL03PA05078
UniParc UPI00000C5EEC
UniProtKB Acc Q9HUA6
UniProtKB ID OPGH_PSEAE
UniRef100 UniRef100_B7V3H0
UniRef50 UniRef50_P20401
UniRef90 UniRef90_B7V3H0

Product

Feature Type CDS
Coding Frame 1
Product Name
OpgH
Product Name Confidence: Class 1
Synonyms MdoH
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Detected using LC-MS/MS (96.99 kDa, PMID:21751344)
Charge (pH 7) 12.13
Kyte-Doolittle Hydrophobicity Value -0.019
Molecular Weight (kDa) 97.0
Isoelectric Point (pI) 8.93

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Membrane Class 3
Outer Membrane Vesicle Class 1
Cytoplasmic Membrane Class 2
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 125 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG004678 (497 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA5077
Search term: opgH
Search term: OpgH

Human Homologs

References

Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res 2005 Feb;15(2):321-9
PubMed ID: 15687295
Molecular analysis of a pathogenicity locus in Pseudomonas syringae pv. syringae.
Mukhopadhyay P, Williams J, Mills D
J Bacteriol 1988 Dec;170(12):5479-88
PubMed ID: 3192509
Homology between a genetic locus (mdoA) involved in the osmoregulated biosynthesis of periplasmic glucans in Escherichia coli and a genetic locus (hrpM) controlling pathogenicity of Pseudomonas syringae.
Loubens I, Debarbieux L, Bohin A, Lacroix JM, Bohin JP
Mol Microbiol 1993 Oct;10(2):329-40
PubMed ID: 7934824
Proteomic analysis of outer membrane vesicles derived from Pseudomonas aeruginosa.
Choi DS, Kim DK, Choi SJ, Lee J, Choi JP, Rho S, Park SH, Kim YK, Hwang D, Gho YS
Proteomics 2011 Aug;11(16):3424-9
PubMed ID: 21751344
Linear osmoregulated periplasmic glucans are encoded by the opgGH locus of Pseudomonas aeruginosa.
Lequette Y, Rollet E, Delangle A, Greenberg EP, Bohin JP
Microbiology (Reading) 2007 Oct;153(Pt 10):3255-3263
PubMed ID: 17906125