Pseudomonas aeruginosa PAO1, PA5111 (gloA3)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA5111
Name
gloA3
Synonym: glo1
Replicon chromosome
Genomic location 5753614 - 5754144 (- strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 2 transposon mutants in orthologs

Cross-References

RefSeq NP_253798.1
GI 15600304
Affymetrix PA5111_gloA3_at
DNASU PaCD00008199
Entrez 882241
GenBank AAG08496.1
INSDC AAG08496.1
NCBI Locus Tag PA5111
protein_id(GenBank) gb|AAG08496.1|AE004924_1|gnl|PseudoCAP|PA5111
TIGR NTL03PA05112
UniParc UPI00000C5F09
UniProtKB Acc Q9HU72
UniProtKB ID LGUL_PSEAE
UniRef100 UniRef100_Q9HU72
UniRef50 UniRef50_Q9HU72
UniRef90 UniRef90_Q9HU72

Product

Feature Type CDS
Coding Frame 1
Product Name
lactoylglutathione lyase
Product Name Confidence: Class 1
Synonyms glyoxalase I
aldoketomutase, methylglyoxalase
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -7.13
Kyte-Doolittle Hydrophobicity Value -0.474
Molecular Weight (kDa) 20.2
Isoelectric Point (pI) 4.77

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 306 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG002141 (841 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA5111
Search term: gloA3

Human Homologs

Ensembl 110, assembly GRCh38.p14
glyoxalase I [Source:HGNC Symbol;Acc:HGNC:4323]
E-value: 4.1e-49
Percent Identity: 57.1

References

The gene encoding glyoxalase I from Pseudomonas putida: cloning, overexpression, and sequence comparisons with human glyoxalase I.
Lu T, Creighton DJ, Antoine M, Fenselau C, Lovett PS
Gene 1994 Dec 2;150(1):93-6
PubMed ID: 7959071
Pseudomonas aeruginosa contains multiple glyoxalase I-encoding genes from both metal activation classes.
Sukdeo N, Honek JF
Biochim Biophys Acta 2007 Jun;1774(6):756-63
PubMed ID: 17513180