Pseudomonas aeruginosa PAO1, PA5119 (glnA)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA5119
Name
glnA
Replicon chromosome
Genomic location 5766484 - 5767893 (+ strand)

Cross-References

RefSeq NP_253806.1
GI 15600312
Affymetrix PA5119_glnA_at
Entrez 877688
GenBank AAG08504.1
INSDC AAG08504.1
NCBI Locus Tag PA5119
protein_id(GenBank) gb|AAG08504.1|AE004925_2|gnl|PseudoCAP|PA5119
TIGR NTL03PA05120
UniParc UPI00000C5F0F
UniProtKB Acc Q9HU65
UniProtKB ID GLNA_PSEAE
UniRef100 UniRef100_Q9HU65
UniRef50 UniRef50_Q3V5W6
UniRef90 UniRef90_Q3V5W6

Product

Feature Type CDS
Coding Frame 1
Product Name
glutamine synthetase
Product Name Confidence: Class 2
Synonyms glutamate--ammonia ligase
Evidence for Translation
Detected using LC-MS/MS (51.94 kDa, PMID:21751344)
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -18.49
Kyte-Doolittle Hydrophobicity Value -0.340
Molecular Weight (kDa) 51.9
Isoelectric Point (pI) 4.95

Subcellular localization

Individual Mappings
Localization Confidence PMID
Outer Membrane Vesicle Class 1
Cytoplasmic Class 2
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 580 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG004712 (536 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA5119
Search term: glnA

Human Homologs

References

Expression of glnA in Escherichia coli is regulated at tandem promoters.
Reitzer LJ, Magasanik B
Proc Natl Acad Sci U S A 1985 Apr;82(7):1979-83
PubMed ID: 2858855
The complete nucleotide sequence of the glnALG operon of Escherichia coli K12.
Miranda-Ríos J, Sánchez-Pescador R, Urdea M, Covarrubias AA
Nucleic Acids Res 1987 Mar 25;15(6):2757-70
PubMed ID: 2882477
Proteomic analysis of outer membrane vesicles derived from Pseudomonas aeruginosa.
Choi DS, Kim DK, Choi SJ, Lee J, Choi JP, Rho S, Park SH, Kim YK, Hwang D, Gho YS
Proteomics 2011 Aug;11(16):3424-9
PubMed ID: 21751344