Pseudomonas aeruginosa PAO1, PA5174 (fabY)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA5174
Name
fabY
Replicon chromosome
Genomic location 5826135 - 5828039 (+ strand)
Transposon Mutants in orthologs 2 transposon mutants in orthologs
CommentFabY is abeta-ketoacyl-acyl carrier protein synthase I/II (KASI/II) family member. P. aeruginosa is unusual among bacterial because this step in bacterial fatty acid synthesis is normally carried out by FabH (a member of the KASIII family).

Cross-References

RefSeq NP_253861.1
GI 15600367
Affymetrix PA5174_at
Entrez 881783
GenBank AAG08559.1
INSDC AAG08559.1
NCBI Locus Tag PA5174
protein_id(GenBank) gb|AAG08559.1|AE004930_5|gnl|PseudoCAP|PA5174
TIGR NTL03PA05175
UniParc UPI00000C5F37
UniProtKB Acc Q9HU15
UniProtKB ID Q9HU15_PSEAE
UniRef100 UniRef100_A0A0C6EST4
UniRef50 UniRef50_S0AA98
UniRef90 UniRef90_B7V3R9

Product

Feature Type CDS
Coding Frame 1
Product Name
beta-acetoacetyl-acyl carrier protein synthase FabY
Product Name Confidence: Class 1
Synonyms beta-ketoacyl-[acyl-carrier-protein] synthase III
Evidence for Translation
Detected by MALDI-TOF/TOF (PMID:19333994).
Detected by iTRAQ (PMID:21863142).
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -8.51
Kyte-Doolittle Hydrophobicity Value -0.134
Molecular Weight (kDa) 68.8
Isoelectric Point (pI) 6.02

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
4CW4 TRANSFERASE 04/01/14 Crystal structure of the noncanonical ketosynthase FabY from P. aeruginosa PSEUDOMONAS AERUGINOSA 1.349 X-RAY DIFFRACTION 100.0

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 568 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG004762 (323 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA5174
Search term: fabY

Human Homologs

References

Twenty-five coregulated transcripts define a sterigmatocystin gene cluster in Aspergillus nidulans.
Brown DW, Yu JH, Kelkar HS, Fernandes M, Nesbitt TC, Keller NP, Adams TH, Leonard TJ
Proc Natl Acad Sci U S A 1996 Feb 20;93(4):1418-22
PubMed ID: 8643646
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994
Comparisons of Two Proteomic Analyses of Non-Mucoid and Mucoid Pseudomonas aeruginosa Clinical Isolates from a Cystic Fibrosis Patient.
Rao J, Damron FH, Basler M, Digiandomenico A, Sherman NE, Fox JW, Mekalanos JJ, Goldberg JB
Front Microbiol 2011;2:162
PubMed ID: 21863142
Fatty acid biosynthesis in Pseudomonas aeruginosa is initiated by the FabY class of β-ketoacyl acyl carrier protein synthases.
Yuan Y, Sachdeva M, Leeds JA, Meredith TC
J Bacteriol 2012 Oct;194(19):5171-84
PubMed ID: 22753059
Pseudomonas aeruginosa directly shunts β-oxidation degradation intermediates into de novo fatty acid biosynthesis.
Yuan Y, Leeds JA, Meredith TC
J Bacteriol 2012 Oct;194(19):5185-96
PubMed ID: 22753057
Deletion of the β-acetoacetyl synthase FabY in Pseudomonas aeruginosa induces hypoacylation of lipopolysaccharide and increases antimicrobial susceptibility.
Six DA, Yuan Y, Leeds JA, Meredith TC
Antimicrob Agents Chemother 2014;58(1):153-61
PubMed ID: 24145528
Will the initiator of fatty acid synthesis in Pseudomonas aeruginosa please stand up?
Zhang YM, Rock CO
J Bacteriol 2012 Oct;194(19):5159-61
PubMed ID: 22821980