Strain |
Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest |
Locus Tag |
PA5174
|
Name |
fabY
|
Replicon | chromosome |
Genomic location | 5826135 - 5828039 (+ strand) |
Transposon Mutants in orthologs | 2 transposon mutants in orthologs |
Comment | FabY is abeta-ketoacyl-acyl carrier protein synthase I/II (KASI/II) family member. P. aeruginosa is unusual among bacterial because this step in bacterial fatty acid synthesis is normally carried out by FabH (a member of the KASIII family). |
RefSeq | NP_253861.1 |
GI | 15600367 |
Affymetrix | PA5174_at |
Entrez | 881783 |
GenBank | AAG08559.1 |
INSDC | AAG08559.1 |
NCBI Locus Tag | PA5174 |
protein_id(GenBank) | gb|AAG08559.1|AE004930_5|gnl|PseudoCAP|PA5174 |
TIGR | NTL03PA05175 |
UniParc | UPI00000C5F37 |
UniProtKB Acc | Q9HU15 |
UniProtKB ID | Q9HU15_PSEAE |
UniRef100 | UniRef100_A0A0C6EST4 |
UniRef50 | UniRef50_S0AA98 |
UniRef90 | UniRef90_B7V3R9 |
Feature Type | CDS |
Coding Frame | 1 |
Product Name |
beta-acetoacetyl-acyl carrier protein synthase FabY
|
Synonyms |
beta-ketoacyl-[acyl-carrier-protein] synthase III |
Evidence for Translation |
Detected by MALDI-TOF/TOF (PMID:19333994).
Detected by iTRAQ (PMID:21863142).
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
|
Charge (pH 7) | -8.51 |
Kyte-Doolittle Hydrophobicity Value | -0.134 |
Molecular Weight (kDa) | 68.8 |
Isoelectric Point (pI) | 6.02 |
Individual Mappings | |
Additional evidence for subcellular localization |
Accession | Header | Accession Date | Compound | Source | Resolution | Method | Percent Identity |
4CW4 | TRANSFERASE | 04/01/14 | Crystal structure of the noncanonical ketosynthase FabY from P. aeruginosa | PSEUDOMONAS AERUGINOSA | 1.349 | X-RAY DIFFRACTION | 100.0 |
Results |
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 568 genera
|
Pseudomonas Ortholog Database | View orthologs at Pseudomonas Ortholog Database |
Pseudomonas Ortholog Group |
POG004762 (323 members) |
Putative Inparalogs | None Found |
STRING database | Search for predicted protein-protein interactions using:
Search term: PA5174
Search term: fabY
Search term: beta-acetoacetyl-acyl carrier protein synthase FabY
|
Twenty-five coregulated transcripts define a sterigmatocystin gene cluster in Aspergillus nidulans.
Brown DW, Yu JH, Kelkar HS, Fernandes M, Nesbitt TC, Keller NP, Adams TH, Leonard TJ
Proc Natl Acad Sci U S A 1996 Feb 20;93(4):1418-22
PubMed ID: 8643646
|
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994
|
Comparisons of Two Proteomic Analyses of Non-Mucoid and Mucoid Pseudomonas aeruginosa Clinical Isolates from a Cystic Fibrosis Patient.
Rao J, Damron FH, Basler M, Digiandomenico A, Sherman NE, Fox JW, Mekalanos JJ, Goldberg JB
Front Microbiol 2011;2:162
PubMed ID: 21863142
|
Fatty acid biosynthesis in Pseudomonas aeruginosa is initiated by the FabY class of β-ketoacyl acyl carrier protein synthases.
Yuan Y, Sachdeva M, Leeds JA, Meredith TC
J Bacteriol 2012 Oct;194(19):5171-84
PubMed ID: 22753059
|
Pseudomonas aeruginosa directly shunts β-oxidation degradation intermediates into de novo fatty acid biosynthesis.
Yuan Y, Leeds JA, Meredith TC
J Bacteriol 2012 Oct;194(19):5185-96
PubMed ID: 22753057
|
Deletion of the β-acetoacetyl synthase FabY in Pseudomonas aeruginosa induces hypoacylation of lipopolysaccharide and increases antimicrobial susceptibility.
Six DA, Yuan Y, Leeds JA, Meredith TC
Antimicrob Agents Chemother 2014;58(1):153-61
PubMed ID: 24145528
|
Will the initiator of fatty acid synthesis in Pseudomonas aeruginosa please stand up?
Zhang YM, Rock CO
J Bacteriol 2012 Oct;194(19):5159-61
PubMed ID: 22821980
|