Pseudomonas aeruginosa PAO1, PA5199 (amgS)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA5199
Name
amgS
Replicon chromosome
Genomic location 5851239 - 5852558 (- strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 3 transposon mutants in orthologs

Cross-References

RefSeq NP_253886.1
GI 15600392
Affymetrix PA5199_envZ_at
Entrez 880300
GenBank AAG08584.1
INSDC AAG08584.1
NCBI Locus Tag PA5199
protein_id(GenBank) gb|AAG08584.1|AE004932_11|gnl|PseudoCAP|PA5199
TIGR NTL03PA05200
UniParc UPI00000C5F4D
UniProtKB Acc Q9HTZ0
UniProtKB ID Q9HTZ0_PSEAE
UniRef100 UniRef100_W0YZZ5
UniRef50 UniRef50_Q4KK10
UniRef90 UniRef90_Q02EG5

Product

Feature Type CDS
Coding Frame 1
Product Name
AmgS
Product Name Confidence: Class 1
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -3.32
Kyte-Doolittle Hydrophobicity Value -0.085
Molecular Weight (kDa) 48.8
Isoelectric Point (pI) 6.20

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Membrane Class 2
Cytoplasmic Membrane Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 620 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG004783 (534 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA5199
Search term: amgS
Search term: AmgS

Human Homologs

References

Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res 2005 Feb;15(2):321-9
PubMed ID: 15687295
Primary characterization of the protein products of the Escherichia coli ompB locus: structure and regulation of synthesis of the OmpR and EnvZ proteins.
Comeau DE, Ikenaka K, Tsung KL, Inouye M
J Bacteriol 1985 Nov;164(2):578-84
PubMed ID: 2997120
Identification of the site of phosphorylation on the osmosensor, EnvZ, of Escherichia coli.
Roberts DL, Bennett DW, Forst SA
J Biol Chem 1994 Mar 25;269(12):8728-33
PubMed ID: 8132603
Targeting a bacterial stress response to enhance antibiotic action.
Lee S, Hinz A, Bauerle E, Angermeyer A, Juhaszova K, Kaneko Y, Singh PK, Manoil C
Proc Natl Acad Sci U S A 2009 Aug 25;106(34):14570-5
PubMed ID: 19706543