Pseudomonas aeruginosa PAO1, PA5221

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA5221
Name
Synonym: visC
Replicon chromosome
Genomic location 5878754 - 5879971 (- strand)
Transposon Mutants 1 transposon mutants in PAO1

Cross-References

RefSeq NP_253908.1
GI 15600414
Affymetrix PA5221_at
Entrez 879231
GenBank AAG08606.1
INSDC AAG08606.1
NCBI Locus Tag PA5221
protein_id(GenBank) gb|AAG08606.1|AE004935_3|gnl|PseudoCAP|PA5221
TIGR NTL03PA05222
UniParc UPI00000C5F5F
UniProtKB Acc Q9HTW9
UniProtKB ID Q9HTW9_PSEAE
UniRef100 UniRef100_A0A0A8RIZ1
UniRef50 UniRef50_D7HUR2
UniRef90 UniRef90_A0A022PAK7

Product

Feature Type CDS
Coding Frame 1
Product Name
probable FAD-dependent monooxygenase
Product Name Confidence: Class 3
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -2.77
Kyte-Doolittle Hydrophobicity Value -0.112
Molecular Weight (kDa) 44.6
Isoelectric Point (pI) 6.70

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Membrane Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 290 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG004803 (535 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA5221

Human Homologs

Ensembl 110, assembly GRCh38.p14
coenzyme Q6, monooxygenase [Source:HGNC Symbol;Acc:HGNC:20233]
E-value: 4.5e-51
Percent Identity: 33.5

References

Isolation and characterization of a light-sensitive mutant of Escherichia coli K-12 with a mutation in a gene that is required for the biosynthesis of ubiquinone.
Nakahigashi K, Miyamoto K, Nishimura K, Inokuchi H
J Bacteriol 1992 Nov;174(22):7352-9
PubMed ID: 1339425